MABS496
Anti-Mineralocorticoid Receptor Antibody, clone 6G1
clone 6G1, from mouse
Synonyme(s) :
MR, Nuclear receptor subfamily 3 group C member 2, Mineralocorticoid Receptor
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
6G1, monoclonal
Espèces réactives
rat, mouse
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
Isotype
IgG1
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
mouse ... Nr3C2(110784)
Description générale
Mineralocorticoid receptor (UniProt P22199; also known as MR, Nuclear receptor subfamily 3 group C member 2) is encoded by the Nr3c2 (also known as MCR, Mlr) gene (Gene ID 25672) in rats. Mineralocorticoid receptor (MR) is expressed in multiple tissues, including renal epithelia, smooth muscle, endothelium, cardiomyocytes, and hippocampal neurons, where it mediates diverse functions. MR is activated by aldosterone and also by cortisol in cells that do not express 11β-hydroxysteroid dehydrogenase type 2 (11βHSD2). MR is normally activated by aldosterone, which is produced by adrenal glomerulosa in response to intravascular volume depletion and hyperkalemia. Consistently, gain of function mutations in MR lead to severe hypertension, often with hypokalemia, while loss of function mutations result in neonatal hypotension. Ser843 phosphorylation in the MR ligand-binding domain is reported to prevent MR ligand binding and activation. MR pS843 is found exclusively in intercalated cells of the distal nephrons in the kidney. Intravascular volume depletion, angiotensin II and WNK4 signaling reduce MR pS843 levels, whereas hyperkalemia increases MR pS843.
Spécificité
Epitope is present in all 3 alternative spliced isoforms.
Immunogène
Conjugated linear peptide corresponding to rat Mineralocorticoid Receptor.
Application
Detect Mineralocorticoid Receptor using this Anti-Mineralocorticoid Receptor Antibody, clone 6G1 validated for use in Western Blotting, Immunohistochemistry and Immunocytochemistry.
Immunohistochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in various rat tissues including kidney, hippocampus, choroid plexus, cerebellum, colon, heart, and coronary artery (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348).
Immunocytochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in mouse kidney cells (Shibata, S., et al. (2013). Cell Metabolism. 18:660-671).
Western Blotting Analysis: A representative lot detected Mineralocorticoid Receptor in rat hippocampal tissue lysate (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348).
Western Blotting Analysis: A representative lot detected endogenous Mineralocorticoid Receptor (MR) in mouse kidney and exogenously expressed human MR in transfected COS-7 cells (Shibata, S., et al. (2013) Cell Metab. 18(5):660-671).
Immunocytochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in mouse kidney cells (Shibata, S., et al. (2013). Cell Metabolism. 18:660-671).
Western Blotting Analysis: A representative lot detected Mineralocorticoid Receptor in rat hippocampal tissue lysate (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348).
Western Blotting Analysis: A representative lot detected endogenous Mineralocorticoid Receptor (MR) in mouse kidney and exogenously expressed human MR in transfected COS-7 cells (Shibata, S., et al. (2013) Cell Metab. 18(5):660-671).
Qualité
Evaluated by Western Blotting in M1MR cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Mineralocorticoid Receptor in 10 µg of M1MR cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Mineralocorticoid Receptor in 10 µg of M1MR cell lysate.
Description de la cible
~107 kDa observed
Forme physique
Format: Purified
Autres remarques
Concentration: Please refer to lot specific datasheet.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Naoual Dahmana et al.
International journal of pharmaceutics, 604, 120773-120773 (2021-06-07)
Impaired wound healing in patients receiving glucocorticoid therapy is a serious clinical concern: mineralocorticoid receptor (MR) antagonists can counter glucocorticoid-induced off-target activation of MR receptors. The aim of this study was to investigate the cutaneous delivery of the potent MR
Viren H Makhijani et al.
Neuropharmacology, 181, 108337-108337 (2020-10-03)
The mineralocorticoid receptor (MR) is an emerging target in the field of alcohol research. The MR is a steroid receptor in the same family as the glucocorticoid receptor, with which it shares the ligand corticosterone in addition to the MR
Serena M Dudek et al.
Hippocampus, 33(6), 700-711 (2023-05-09)
Since 1959, the Russian Farm-Fox study has bred foxes to be either tame or, more recently, aggressive, and scientists have used them to gain insight into the brain structures associated with these behavioral features. In mice, hippocampal area CA2 has
Mahshid Gazorpak et al.
Nature communications, 14(1), 8177-8177 (2023-12-10)
Counteracting the overactivation of glucocorticoid receptors (GR) is an important therapeutic goal in stress-related psychiatry and beyond. The only clinically approved GR antagonist lacks selectivity and induces unwanted side effects. To complement existing tools of small-molecule-based inhibitors, we present a
Jakob Hartmann et al.
Cell reports, 35(9), 109185-109185 (2021-06-03)
Responding to different dynamic levels of stress is critical for mammalian survival. Disruption of mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) signaling is proposed to underlie hypothalamic-pituitary-adrenal (HPA) axis dysregulation observed in stress-related psychiatric disorders. In this study, we show
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