MABS351

Anti-FAT10 Antibody, clone 4F1

clone 4F1, from mouse

• Synonyme(s) : Ubiquitin D, Diubiquitin, Ubiquitin-like protein FAT10
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 4F1, monoclonal
• Espèces réactives: rat, mouse, human
• Technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG2a
• Numéro d'accès NCBI: NP_006389
• Numéro d'accès UniProt: O15205
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... UBD(10537)

Description

Description générale

FAT10 protein is a recombinant protein produced in E. coli corresponding to the human sequence encompassing an N-terminal His6-tag within a leader sequence. FAT10, a ubiquitin-like protein, is encoded in the major histocompatibility complex class I locus and is synergistically inducible with interferon-gamma and tumor necrosis factor alpha. FAT10 expression causes apoptosis and it appears that FAT10 may modulate tumorigenesis through its interaction with the MAD2 spindle-assembly checkpoint protein. It has been demonstrated that FAT10 and its conjugates are rapidly degraded by the proteasome. Furthermore, a new interaction partner of FAT10, NEDD8 ultimate buster-1L (NUB1L), may function as a linker that targets FAT10 for degradation by the proteasome.

Immunogène

GST-tagged recombinant protein corresponding to human FAT10.

Application

Anti-FAT10 Antibody, clone 4F1 is a highly specific mouse monoclonal antibody, that targets FAT10 & has been tested in western blotting, IP & ICC.

Immunocytochemistry Analysis: A representative lot detected FAT10 in HA-FAT10 transfected HEK293 cells (Aichem, A., et al. (2010). Nature Commun. 1:13).
Immunoprecipitation Analysis: A representative lot immunoprecipitated FAT10 in transfected HA-FAT10 HEK293 cells cultivated with IFNgamma and TNFalpha (Aichem, A., et al. (2010). Nature Commun. 1:13).

Qualité

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected FAT10 in 10 µg of HepG2 cell lysate.

Description de la cible

~18 kDa observed. FAT10 FAT10ylates itself as well as other proteins so additional bands may be observed due to the FAT10ylation of endogenous proteins (Aichem, A., et al. (2010). Nature Commun. 1:13.),(Aichem, A., et al. J Cell Sci. (2012)125:4576-85).

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable