MABN819
Anti-Tau Antibody, oligomeric Antibody, clone TOMA-1
clone TOMA-1, from mouse
Synonyme(s) :
Microtubule-associated protein tau oligomer, Tau oligomer, PHF-tau oligomer, Paired helical filament-tau oligomer, Neurofibrillary tangle protein oligomer
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
TOMA-1, monoclonal
Espèces réactives
rat, human, mouse
Réactivité de l'espèce (prédite par homologie)
mammals (based on high homology)
Technique(s)
ELISA: suitable
dot blot: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
neutralization: suitable
western blot: suitable
Isotype
IgG2aκ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
ambient
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... MAPT(4137)
mouse ... Mapt(17762)
rat ... Mapt(29477)
Description générale
Microtubule-associated protein tau (UniProt P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as TAU, MAPT1, MTBTL) gene (Gene ID 4137) in human. In Alzheimer′s disease (AD) pathology, accumulation of the microtubule-associated protein tau takes place primarily in the neurons. Tau accumulates in both the somatodendritic and axonal domains of neurons. Tau also accumulates in the soma as neurofibrillary tangles (NFTs). Cell death and synaptic lesions occur independently of NFT formation, and research indicates that NFT formation alone is insufficient for neurodegeneration, suggesting that soluble tau aggregates may be the more toxic and pathologically significant tau species. Tau oligomers are neurotoxic when applied extracellularly to cultured neuronal cells, and tau oligomers (but not fibrils) induce neurodegeneration and synaptic and mitochondrial dysfunction in vivo. Moreove, researchers are able to use tau oligomers as a reliable biomarker to differentiate AD brains from age-matched non-AD brains.
Spécificité
Clone TOMA-1 (a.k.a. clone H12C10) is among more than 13 hybridomas derived from mice immunized with in vitro generated recombinant Tau-441 (Tau-F, Tau-4, 2N4R isoform) oligomers. These TOMAs are reported to specifically detect oligomeric tau without any significant reactivity toward monomeric tau, tau fibrils, Aβ oligomers, Aβ fibrils, or α-synuclein oligomers (Castillo-Carranza, D.L., et al. (2014). J. Neurosci. 34(12):4260-4272).
Immunogène
Recombinant human Tau-441 (Tau-F, Tau-4, 2N4R isoform) oligomers (Lasagna-Reeves, C. A., et al. (2012). FASEB J. 26(5):1946-1959).
Application
Anti-Tau, oligomeric, clone TOMA-1, Cat. No. MABN819, is amouse monoclonal antibody that targets tau oligomers and has been tested in Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry, Neutralization, and Western Blotting applications.
Research Category
Neuroscience
Neuroscience
Western Blotting Analysis: 20 µg/mL from a representative lot detected upregulated oligomeric tau in soluble PBS brain extract from Alzheimer′s diseased (AD) human brain (Courtesy of Sengupta, U., et al., Kayed lab, University of Texas, Galveston).
Please refer to the following publications regarding the use of TOMA clones in Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry, Neutralization, and Western Blotting applications:
1. Vuono, R., et al. (2015). Brain. 138(Pt 7):1907-1918.
2. Castillo-Carranza, D.L., et al. (2015). J. Neurosci. 35(12):4857-4868.
3. Castillo-Carranza, D.L., et al. (2014). J. Neurosci. 34(12):4260-4272.
Please refer to the following publications regarding the use of TOMA clones in Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry, Neutralization, and Western Blotting applications:
1. Vuono, R., et al. (2015). Brain. 138(Pt 7):1907-1918.
2. Castillo-Carranza, D.L., et al. (2015). J. Neurosci. 35(12):4857-4868.
3. Castillo-Carranza, D.L., et al. (2014). J. Neurosci. 34(12):4260-4272.
Qualité
Evaluated by Western Blotting in Alzheimer′s diseased human brain lysate.
Western Blotting Analysis: 4 µg/mL of this antibody detected Tau oligmers in 25 µg of Alzheimer′s diseased human brain lysate.
Western Blotting Analysis: 4 µg/mL of this antibody detected Tau oligmers in 25 µg of Alzheimer′s diseased human brain lysate.
Description de la cible
Variable depending on the size(s) of the oligomer(s). Uncharacterized bands may be observed in some lysate(s).
Forme physique
Format: Purified
Protein A purified.
Purified mouse IgG2aκ in buffer containing PBS without preservatives.
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Yan Zhu et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 38(48), 10255-10270 (2018-10-17)
Brainstem locus ceruleus neurons (LCn) are among the first neurons across the lifespan to evidence tau pathology, and LCn are implicated in tau propagation throughout the cortices. Yet, events influencing LCn tau are poorly understood. Activated persistently across wakefulness, LCn
Urmi Sengupta et al.
Annals of clinical and translational neurology, 4(4), 226-235 (2017-04-07)
With an increasing incidence of Alzheimer's disease (AD) and neurodegenerative tauopathies, there is an urgent need to develop reliable biomarkers for the diagnosis and monitoring of the disease, such as the recently discovered toxic tau oligomers. Here, we aimed to
Urmi Sengupta et al.
Methods in molecular biology (Clifton, N.J.), 2754, 147-183 (2024-03-21)
Tau oligomers have been shown to be the main toxic tau species in several neurodegenerative disorders. To study tau oligomers, we have developed reagents and established methods for the reliable preparation, isolation, and detection of tau oligomers as well as
Fan Xia et al.
Acta neuropathologica communications, 9(1), 51-51 (2021-03-26)
The retina, as the only visually accessible tissue in the central nervous system, has attracted significant attention for evaluating it as a biomarker for neurodegenerative diseases. Yet, most of studies focus on characterizing the loss of retinal ganglion cells (RGCs)
Fang Du et al.
Brain : a journal of neurology, 146(10), 4378-4394 (2023-04-19)
Prolonged exposure to glucocorticoids, the main stress hormones, damages the brain and is a risk factor for depression and Alzheimer's disease. Two major drivers of glucocorticoid-related neurotoxicity are mitochondrial dysfunction and Tau pathology; however, the molecular/cellular mechanisms precipitating these events
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