MABN1544

Anti-IP3R3 Antibody, clone 6G12.1

clone 6G12.1, from mouse

• Synonyme(s) : Inositol 1, 4, 5-trisphosphate receptor type 3, InsP3R3, IP3 receptor isoform 3, IP3R 3, Type 3 inositol 1, 4, 5-trisphosphate receptor, Type 3 InsP3 receptor
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 6G12.1, monoclonal
• Espèces réactives: human
• Technique(s): western blot: suitable
• Isotype: IgG2bκ
• Numéro d'accès NCBI: NP_002215
• Numéro d'accès UniProt: Q14573
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ITPR3(3710)

Description

Description générale

InsP3R3, IP3 receptor isoform 3, IP3R 3, Type 3 inositol 1,4,5-trisphosphate receptor, Type 3 InsP3 receptor) is encoded by the ITPR3 gene (Gene ID 3710) in human. The inositol 1,4,5-trisphosphate (InsP3) receptors, InsP3R1, InsP3R2, and InsP3R3, play an important role in maintaining normal epithelial secretory function by mediating intracellular Ca2+ release. InsP3R3 is the major isoform in hepatic bile ducts, where it is concentrated along the subapical portion of the ER and regulates bicarbonate (HCO3 ) and chloride (Cl ) secretion into the bile. Wherease hepatocytes express mainly InsP3R2 that is involved in modulating the secretion of bile solutes. A loss of InsP3R3 expression in cholangiocytes is found in primary biliary cirrhosis (PBC), an autoimmune liver disease characterized by the loss of bile ducts and impaired bile secretion. Likewise, InsP3R3 and InsP3R2 double knockout mice are reported to exhibit severe defects of pancreatic zymogen secretion. Human InsP3R3 is a 6-transmembrane (a.a. 2203-2223, 2236-2256, 2265-2285, 2326-2346, 2369-2389, 2497-2517) ER membrane protein, having both its N- and C-terminal ends (a.a. 1-2202 and 2518-2671) exposed at the cytoplasmic side. The N-terminal cytoplasmic region contains five MIR (Mannosyltransferase, InsP3R, RyR) domains (a.a. 113-173, 174-224, 232-288, 295-372, 378-434) and the InsP3-binding sites (a.a. 266-270, 507-510, 567-569).

Spécificité

Clone 6G12.1 targets an epitope in the C-terminal half of human IP3R3 (IP3 Receptor 3) N-terminal cytoplasmic domain.

Immunogène

GST-tagged recombinant human IP3R3 (IP3 Receptor 3) N-terminal cytoplasmic domain fragment.

Application

Anti-IP3R3 Antibody, clone 6G12.1, Cat. No. MABN1544, is a highly specific mouse monoclonal antibody that targets ITPR3 and has been tested in Western Blotting.

Research Category
Neuroscience

Qualité

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected IP3R3 (IP3 Receptor 3) in 10 µg of HeLa cell lysate.

Description de la cible

~260 kDa observed. 304.1 kDa calculated.

Forme physique

Format: Purified

Protein G purified.

Purified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable