MABF2786

Anti-IL-9 Antibody, clone MH9A3

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.74

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: MH9A3, monoclonal
• Poids mol.: calculated mol wt 15.91 kDa
• Produit purifié par: using protein G
• Espèces réactives: human
• Conditionnement: antibody small pack of 100 μL
• Technique(s): ELISA: suitable; flow cytometry: suitable; multiplexing: suitable; neutralization: suitable
• Isotype: IgG1
• Séquence de l'épitope: Unknown
• Numéro d'accès Protein ID: NP_000581
• Numéro d'accès UniProt: P15248
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... il9> IL9(3578)

Description

Description générale

Interleukin-9 (UniProt: P15248; also known as IL-9, Cytokine P40, T-cell growth factor P40) is encoded by the IL9 gene (Gene ID: 3578) in human. IL-9 is a pleiotropic cytokine that plays a significant role in tolerance and inflammation. It is expressed by a variety of immune cells. It is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form. It supports the growth of mast cells, T cells, and several other cell types, including erythroid progenitors, fetal thymocytes, human megakaryoblastic leukemic cells, and myeloid precursor cells. It is also reported to supports IL-2 independent and IL-4 independent growth of helper T-cells. It also plays a role in promoting IgE production by B cells. IL-9 actions are mediated via its binding to IL-9R that results in the formation the IL-9R heterocomplex. Following its binding to the IL-9R , the SHC is phosphorylated, which then binds to Grb2 that subsequently activates SoS, a GTP exchange factor for Ras GTPases. This results in the activation of Ras GTPase that activates Raf kinase followed by the activation of MEK1/2 and ERK1/2. Although IL-9 is reported to activate the MAPK pathway in several lymphoid and hematopoietic cells, this activation is weaker compared to other cytokines. Elevated levels of IL-9 and IL-9R are observed in lungs of asthmatic subjects. (Ref.: Chakraborty, S., et al. (2019). Int. J. Mol. Sci. 20(9); 2113).

Spécificité

Clone MH9A3 is a mouse monoclonal antibody that detects human Interleukin-9.

Immunogène

Ovalbumin-conjugated recombinant human Interleukin-9.

Application

Quality Control Testing

Evaluated by ELISA with recombinant human IL-9.

ELISA Analysis: Various dilution of this antibody detected recombinant human Interleukin-9 (IL-9).

Tested Applications

ELISA Analysis: A representative lot detected IL-9 in ELISA applications (Jenmalm, M.C., et al. (2001). Clin Exp Allergy.;31(10):1528-35; Faulkner, H., et al. (2002). J Infect Dis.;185(5):665-72; Bottcher, M.F., et al. (2003). Pediatr Allergy Immunol.;14(5):345-50; Holz, L.E., et al. (2005). J Inflamm (Lond).;2:3.; Stephens, G.L., et al. (2011). Eur J Immunol.;41(4):952-62; Muls, N., et al. (2012). J Neuroimmunol.;243(1-2):73-80; Peters, C., et al. (2018). Oncoimmunology.;8(1):e1522471).

Flow Cytometry Analysis: A representative lot detected IL-9 in Flow Cytometry applications (Stephens, G.L., et al. (2011). Eur J Immunol.;41(4):952-62; Peters, C., et al. (2018). Oncoimmunology.;8(1):e1522471).

Multiplexing: A representative lot detected IL-9 in Multiplexing applications (Peters, C., et al. (2018). Oncoimmunology.;8(1):e1522471).

Neutralizing: A representative lot neutralized the action of IL-9 in Neutralizing applications (Stephens, G.L., et al. (2011). Eur J Immunol.;41(4):952-62).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-IL-9, clone MH9A3, Cat. No. MABF2786, is a mouse monoclonal antibody that detects Interleukin-9 and is tested for use in ELISA, Flow Cytometry, Multiplexing, and Neutralizing.

Forme physique

Purified mouse monoclonal antibody IgG1 in buffer containing PBS without preservatives.

Stockage et stabilité

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable