MABC991

Anti-PD-L1 Antibody, clone 10F.9G2

clone 10F.9G2, from rat

• Synonyme(s) : PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rat
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 10F.9G2, monoclonal
• Espèces réactives: mouse
• Technique(s): flow cytometry: suitable; immunohistochemistry: suitable; inhibition assay: suitable
• Isotype: IgG2bκ
• Numéro d'accès NCBI: NP_068693
• Numéro d'accès UniProt: Q9EP73
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

Programmed cell death 1 ligand 1 (UniProt: Q9EP73; also known as PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274) is encoded by the CD274 (also known as B7h1, Pdcd1l1, Pdcd1lg1, Pdl1) gene (Gene ID: 60533) in murine species. PD-1 and PD-1 ligands 1 and 2 (PD-L1 and PD-L2) are B7:CD28 family members that regulate T cell activation and peripheral tolerance. PD-L1 is initially produced with signal peptide (aa: 1-18) sequence, the removal of which yields the mature protein with a large extracellular (aa: 19-238) region that contains an Ig-like V-type domain (aa: 19-127) and an Ig-like C2-type domain (aa: 133-225), followed by a transmembrane domain (aa: 239-259) and a cytoplasmic tail (aa: 260-290). When engaged together with the TCR, the interaction of PD-1 with its ligands delivers an inhibitory signal to T cell proliferation and cytokine production. While PD-L1 is broadly expressed in hematopoietic and non-hematopoietic cells, PD-L2 expression is highly restricted to antigen presenting cells (APCs), including dendritic cells (DCs) and macrophages. The PD-1 pathway plays a key role in the progressive loss of effector T cell responses during chronic HIV infection. Tumor-associated PD-L1 is reported to facilitate apoptosis of activated T cells and stimulate IL-10 production in T cells to mediate immune suppression. These effects can be blocked by some monoclonal PD-L1 antibodies and T cell function can be restored. (Ref.: Chen, L., and Han, X (2015). J. Clin. Invest. 125(9): 3384-3391).

Spécificité

Clone 10F.9G2 specifically detects PD-L1 in murine EL4 lymphoma cells and targets the extracellular domain.

Immunogène

CHO-mPD-L1 transfectants.

Application

Detect PD-L1 using this rat monoclonal Anti-PD-L1 Antibody, clone 10F.9G2, Cat. No. MABC991. It has been tested in Flow Cytometry, Function Induction, Immunohistochemistry, and Inhibition studies.

Immunohistochemistry Analysis: A representative lot detected PD-L1 in Immunohistochemistry applications (Grabie, N., et. al. (2007). Circulation. 116(18):2062-71).

Induces Function Analysis: Blockade with a representative lot of anti-PD-L1 combined with IL-2 enhanced antiviral CD8 T cell responses during chronic LCMV infection (West, E.E., et. al. (2013). J Clin Invest. 123(6):2604-15).

Inhibition Analysis: A representative lot blocked the action of PD-L1 (Grabie, N., et. al. (2007). Circulation. 116(18):2062-71;West, E.E., et. al. (2013). J Clin Invest. 123(6):2604-15;El Annan, J., et. al. (2010). Invest Ophthalmol Vis Sci. 51(7):3418-23).

Qualité

Evaluated by Flow Cytometry in EL4 cells.

Flow Cytometry Analysis: 1 µg/mL of this antibody detected PD-L1 in one million EL4 cells.

Description de la cible

32.78 kDa calculated.

Liaison

Replaces: MABF405

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable