MABC91
Anti-Atg18 (WIPI-2) Antibody, clone 2A2
clone 2A2, from mouse
Synonyme(s) :
WD repeat domain phosphoinositide-interacting protein 2, WIPI-2, WIPI49-like protein 2
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
2A2, monoclonal
Espèces réactives
human
Technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotype
IgG1κ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... WIPI2(26100)
Description générale
Atg18, also known as WIPI-2, is involved in autophagy by regulating the assembly of mulitprotein complexes by presenting a 7-bladed propeller structure for interactions with phospholipids. Atg18 is a mammalian effector of PtdIns3P and is ubiquitously expressed in a variety of cell lines. Studies show that Atg18 is recruited to early autophagosomal structural formation along with Atg16 and ULK1, and Atg18 is required for the formation of LC3-positive autophagosomes.
Spécificité
This antibody recognizes Atg18 at the C-terminus.
Immunogène
Epitope: C-terminus
Linear peptide corresponding to the C-terminus of human Atg18.
Application
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Atg18 in human colorectal adenocarcinoma tissue.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-Atg18 (WIPI-2) Antibody, clone 2A2 is validated for use in WB, IH(P) for the detection of Atg18 (WIPI-2).
Qualité
Evaluated by Western Blot in human testis tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Atg18 in 10 µg of human testis tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Atg18 in 10 µg of human testis tissue lysate.
Description de la cible
~49 kDa observed.
6 isoforms exist with molecular weights ranging from 42 kDa to 48 kDa
6 isoforms exist with molecular weights ranging from 42 kDa to 48 kDa
Forme physique
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Remarque sur l'analyse
Control
Human testis tissue lysate
Human testis tissue lysate
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Shusaku Shibutani et al.
The Journal of biological chemistry, 292(44), 18052-18061 (2017-08-16)
The mechanistic target of rapamycin complex 1 (mTORC1) is a master regulator of protein synthesis and potential target for modifying cellular metabolism in various conditions, including cancer and aging. mTORC1 activity is tightly regulated by the availability of extracellular amino
Taryn J Olivas et al.
The Journal of cell biology, 222(7) (2023-04-28)
As the autophagosome forms, its membrane surface area expands rapidly, while its volume is kept low. Protein-mediated transfer of lipids from another organelle to the autophagosome likely drives this expansion, but as these lipids are only introduced into the cytoplasmic-facing
Takahiro Shimizu et al.
Human molecular genetics, 32(16), 2623-2637 (2023-06-26)
β-propellers that bind polyphosphoinositides (PROPPINs) are an autophagy-related protein family conserved throughout eukaryotes. The PROPPIN family includes Atg18, Atg21 and Hsv2 in yeast and WD-repeat protein interacting with phosphoinositides (WIPI)1-4 in mammals. Mutations in the WIPI genes are associated with
Jie Li et al.
Cellular and molecular gastroenterology and hepatology, 17(6), 1007-1024 (2024-02-10)
In the classic form of α1-antitrypsin deficiency (ATD), the misfolded α1-antitrypsin Z (ATZ) variant accumulates in the endoplasmic reticulum (ER) of liver cells. A gain-of-function proteotoxic mechanism is responsible for chronic liver disease in a subgroup of homozygotes. Proteostatic response
Hayden Weng Siong Tan et al.
Nature communications, 13(1), 3720-3720 (2022-06-29)
PINK1-Parkin mediated mitophagy, a selective form of autophagy, represents one of the most important mechanisms in mitochondrial quality control (MQC) via the clearance of damaged mitochondria. Although it is well known that the conjugation of mammalian ATG8s (mATG8s) to phosphatidylethanolamine
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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