MABC605

Anti-TXNIP Antibody, clone 3A7.1

clone 3A7.1, from mouse

• Synonyme(s) : Thioredoxin-interacting protein, Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 3A7.1, monoclonal
• Espèces réactives: mouse, human
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Isotype: IgG2bκ
• Numéro d'accès NCBI: NP_006463
• Numéro d'accès UniProt: Q9H3M7
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TXNIP(10628)

Description

Description générale

Thioredoxin-interacting protein (UniProt Q9H3M7; also known as Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1) is encoded by the TXNIP (also known as VDUP1) gene (Gene ID 10628) in human. Thioredoxin interacting protein (TXNIP) binds thioredoxin active cysteine residue and suppresses its antioxidant activity. TXNIP overexpression results in increased cellular ROS level and oxidative cell death, while TXNIP suppression enhances cellular resistance to oxidative injury. TXNIP is also shown to inhibit cellular glucose uptake by downregulating glucose transporter 1 (GLUT1) plasma membrane localization, and TXNIP expression is found to be markedly suppressed in many cancers, making TXNIP a potential anticancer therapeutic target.

Spécificité

Clone 3A7.1 targets an epitope within the N-terminal half of TXNIP. Cross-reactivity to human and mouse spliced isoform 2 is likely, but has not been confirmed.

Immunogène

GST-tagged recombinant fragment from the N-terminal half of human TXNIP.

Application

Anti-TXNIP Antibody, clone 3A7.1 is an antibody against TXNIP for use in Western Blotting, Immunocytochemistry.

Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected TXNIP in HUVECs, A431, HeLa, and NIH/3T3 cells.

Qualité

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TXNIP in 10 µg of K562 cell lysate.

Description de la cible

~44 kDa observed. 43.66/37.32 kDa (human isoform 1/2) and 44.36/44.29 kDa (mouse isoform 1/2) calculated.

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable