Accéder au contenu
Merck
Toutes les photos(1)

Documents

MABC1823

Sigma-Aldrich

Anti-CD271/p75NTR Antibody, clone G4B1

Synonyme(s) :

CD271, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Tumor necrosis factor receptor superfamily member 16, p75 ICD

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

G4B1, monoclonal

Poids mol.

calculated mol wt 45.18 kDa

Produit purifié par

using protein G

Espèces réactives

human

Conditionnement

antibody small pack of 100

Technique(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

Isotype

IgG2aκ

Séquence de l'épitope

Extracellular domain

Numéro d'accès Protein ID

Numéro d'accès UniProt

Température de stockage

2-8°C

Informations sur le gène

human ... NGFR(4804)

Spécificité

Clone G4B1 is a mouse monoclonal antibody that detects CD271 (p75ICD). It targets an epitope within the fourth cysteine-rich domain in the extracellular domain.

Immunogène

A recombinant fragment corresponding to the extracellular domain of human CD271/p75NTR.

Application

Quality Control Testing

Evaluated by Flow Cytometry in HPCM2 cells.

Flow Cytometry Analysis: 1.0 µg of this antibody detected CD271/p75NTR in one million HPCM2 cells.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected CD271 in Immunocytochemistry applications (Fujii, K., et al. (2022). Cancer Sci. 113(8):2878-2887).

Flow Cytometry Analysis: A representative lot detected CD271 in Flow Cytometry applications (Fujii, K., et al. (2022). Cancer Sci. 113(8):2878-2887).

Immunohistochemistry Applications: A representative lot detected CD271/p75NTR in Immunohistochemistry applications (Fujii, K., et al. (2022). Cancer Sci. 113(8):2878-2887).

Western Blotting Analysis: A representative lot detected CD271 in Western Blotting applications (Fujii, K., et al. (2022). Cancer Sci. 113(8):2878-2887).

ELISA Analysis: A representative lot detected CD271 in ELISA applications (Fujii, K., et al. (2022). Cancer Sci. 113(8):2878-2887).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Tumor necrosis factor receptor superfamily member 16 (UniProt: P08138; also known as Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, p75 ICD, CD271) is encoded by the NGFR (also known as TNFRSF16) gene (Gene ID: 4804) in human. CD271 is single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 29-250), a transmembrane domain (aa 251-272), and a cytoplasmic domain (aa 273-427). It serves as a low affinity receptor that can bind to NGF, BDNF, NTF3, and NTF4. It forms a heterodimeric receptor with sortilin related VPS10 domain containing receptor 2 (SorCS2) that binds the precursor forms of NGF, BDNF and NTF3 with high affinity, but has much lower affinity for mature NGF and BDNF. CD271 plays an important role in differentiation and survival of specific neuronal populations during development. It can mediate cell survival as well as cell death of neural cells. During development, it signals toward apoptosis to eliminate unnecessary neurons. Its death domain is localized to amino acids 344-421, which is responsible for its interaction with RAN-binding protein 9 (RANBP9). Neurotrophin binding to p75 can activate the MAP kinase pathway leading to cell survival or apoptosis depending on the cell type or ligand type. Clone G4B1 is shown to bind to the fourth cysteine-rich domain (CRD4) in the extracellular domain and is specifically detects glycosylated CD271. It recognizes CD271 expressed in esophageal cancer, but not in normal esophageal basal cells. It also detects the fetal esophageal epithelium and Barrett′s esophagus that possess stem cell-like characteristics. (Ref.: Fujii, K., et al. (2022). Cancer Sci. 113(8); 2878-2887; Glerup, S., et al. (2014). Neuron. 82(5); 1074-1087; Baeza-Raja , B., et al. (2013). J Neurosci. 33(25); 10221-10234).

Forme physique

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommended storage: +2°C to +8°C.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique