MAB4037
Anti-PTEN Antibody, CT, clone A2b1
clone A2b1, Chemicon®, from mouse
Synonyme(s) :
MMAC1, TEP1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
A2b1, monoclonal
Espèces réactives
human, rat, mouse
Fabricant/nom de marque
Chemicon®
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotype
IgG1
Adéquation
not suitable for immunohistochemistry (Paraffin)
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... PTEN(5728) , TEP1(7011)
Spécificité
Specifically recognizes PTEN*, a tumor suppressor protein located on human chromosome 10 (Steck, ′97; Li, ′97; Rhei, ′97) that is often mutated in various types of advanced cancers (Rhei, ′97). The PTEN protein exhibits protein phosphatase activity (Myers, ′97) and can suppress the growth of glioma cells (Furnari, ′97). The antibody recognizes human and mouse PTEN and is specific for the C-terminus. Other species not tested. Reactivity with human fibroblasts, breast epithelial and lung cancer cell lines has been confirmed by western blot, and antibody specificity has been confirmed by peptide blocking studies.
*PTEN is covered under U.S. patents 6,262,242, 6,482,795 and U.S. patent application 10/299,003.
*PTEN is covered under U.S. patents 6,262,242, 6,482,795 and U.S. patent application 10/299,003.
Immunogène
Anti-PTEN was generated by immunization with a 10 amino acid peptide corresponding to amino acids 387-400 near the C-terminus of human PTEN.
Epitope: C-terminus
Application
Anti-PTEN Antibody, C-terminus, clone A2b1 is an antibody against PTEN for use in IC, IH, IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
PI3K, Akt, & mTOR Signaling
Western blot 1:100 (55 kDa)
Immunocytochemistry
Immunohistochemistry: 1:20 to 1:50 for acetone-fixed, fresh frozen tissue. Not effective for paraffin sections.
Immunoprecipitation 1:10 to 1:20
Protocol for immunohistochemistry of frozen tissue sections on glass slides.
1. Fix sections for 5 minutes with cold acetone.
2. Air dry
3. Wash sections with PBS
4. Add blocking solution (horse serum) for 20 minutes
5. Add diluted PTEN antibody and incubate for 1 hour at room temperature.
6. Wash with PBS
7. Add biotinylated secondary antibody (horse anti-mouse, or equivalent).
8. Incubate for 30 minutes at room temperature.
9. Wash with PBS
10. Detect with ABC (Avidin-Peroxidase-Complex) or equivalent.
11. Wash with PBS
12. Develop with DAB substrate.
13. Hematoxylin dye may be used as a counterstain for visualizing nuclei.Breast tumor tissue gives good staining. Prostate tissue does not stain.Optimal working dilutions must be determined by end user.
Immunocytochemistry
Immunohistochemistry: 1:20 to 1:50 for acetone-fixed, fresh frozen tissue. Not effective for paraffin sections.
Immunoprecipitation 1:10 to 1:20
Protocol for immunohistochemistry of frozen tissue sections on glass slides.
1. Fix sections for 5 minutes with cold acetone.
2. Air dry
3. Wash sections with PBS
4. Add blocking solution (horse serum) for 20 minutes
5. Add diluted PTEN antibody and incubate for 1 hour at room temperature.
6. Wash with PBS
7. Add biotinylated secondary antibody (horse anti-mouse, or equivalent).
8. Incubate for 30 minutes at room temperature.
9. Wash with PBS
10. Detect with ABC (Avidin-Peroxidase-Complex) or equivalent.
11. Wash with PBS
12. Develop with DAB substrate.
13. Hematoxylin dye may be used as a counterstain for visualizing nuclei.Breast tumor tissue gives good staining. Prostate tissue does not stain.Optimal working dilutions must be determined by end user.
Description de la cible
55 kDa
Liaison
Replaces: 04-409
Forme physique
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified
Stockage et stabilité
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Remarque sur l'analyse
Control
Breast tumor tissue
Purified active kinase is Catalogue Number 14-488
Breast tumor tissue
Purified active kinase is Catalogue Number 14-488
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Delira Robbins et al.
FEBS letters, 586(23), 4108-4113 (2012-11-06)
Our recent studies suggest a unique role of p53 during the early stage of cancer development. However, how p53 activation is regulated during TPA treatment remains elusive. We used murine skin epidermal JB6 promotion-sensitive (P+) and promotion resistant (P-) cells
Carsten B Schmidt-Weber et al.
European journal of immunology, 32(4), 1196-1204 (2002-04-05)
The phosphatidylinositol phosphatase gene PTEN is a dual specific phosphatase acting on phospho amino acids but also on three phosphorylated inositol phospholipids. Present results demonstrate that PTEN is inducible by costimulatory signals in human CD4(+) T cells. PTEN expression was
Danyu Cui et al.
Molecular medicine reports, 16(5), 7813-7820 (2017-09-26)
A number of short noncoding microRNAs (miRs) have been demonstrated to be highly expressed in many kidney diseases such as renal cancer and lupus nephritis (LN); however, these results have not been extensively investigated. The aim of the present study
Janice B B Lam et al.
PloS one, 4(3), e4968-e4968 (2009-03-26)
Adiponectin is an adipokine possessing beneficial effects on obesity-related medical complications. A negative association of adiponectin levels with breast cancer development has been demonstrated. However, the precise role of adiponectin deficiency in mammary carcinogenesis remains elusive. In the present study
Joseph O Humtsoe et al.
Molecular and cellular biology, 30(7), 1593-1606 (2010-02-04)
Endothelial cell (EC) migration, cell-cell adhesion, and the formation of branching point structures are considered hallmarks of angiogenesis; however, the underlying mechanisms of these processes are not well understood. Lipid phosphate phosphatase 3 (LPP3) is a recently described p120-catenin-associated integrin
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