MAB374-AF488

Anti-GAPDH Antibody, clone 6C5, Alexa Fluor^™ 488 conjugate

clone 6C5, from mouse, ALEXA FLUOR^™ 488

• Synonyme(s) : Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conjugué: ALEXA FLUOR^™ 488
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 6C5, monoclonal
• Espèces réactives: mouse, human
• Réactivité de l'espèce (prédite par homologie): cat (based on 100% sequence homology), fish (based on 100% sequence homology), canine (based on 100% sequence homology), porcine (based on 100% sequence homology), rat (based on 100% sequence homology), rabbit (based on 100% sequence homology)
• Technique(s): immunocytochemistry: suitable
• Isotype: IgG1
• Numéro d'accès NCBI: NM_001082253.1
• Numéro d'accès UniProt: P46406
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... GAPDH(2597) ; mouse ... Gapdh(14433) ; rabbit ... Gapdh(100009074)

Description

Description générale

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Its binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP). As a ′house-keeping′ enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic role in metabolism, it is also involved in the initial stages of apoptosis or oxidative stress response where it is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH can also bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders, including the beta-amyloid precursor protein and the Huntingtin protein where reduced function of GAPDH is associated with Alzheimer′s and Huntington′s disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity.

Spécificité

It has been reported that this antibody does not react with GAPDH from E. coli.

Application

This Anti-GAPDH Antibody, clone 6C5, Alexa Fluor^™ 488 conjugate is validated for use in Immunocytochemistry for the detection of GAPDH.

Qualité

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected GAPDH in A431 cells.

Description de la cible

~38 kDa observed

Forme physique

Purified mouse conjugate antibody in buffer containing PBS 15 mg/ml BSA and 0.1% Sodium Azide.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations légales

ALEXA FLUOR is a trademark of Life Technologies

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable