MAB3199Z-C

Anti-E-Cadherin Antibody, clone 67A4, Azide & Ascites Free

clone 67A4, 0.5 mg/mL, from mouse

• Synonyme(s) : Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 67A4, monoclonal
• Espèces réactives: human
• Concentration: 0.5 mg/mL
• Technique(s): flow cytometry: suitable; immunofluorescence: suitable; inhibition assay: suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_004351
• Numéro d'accès UniProt: P12830
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CDH1(999)

Description

Description générale

Cadherins are a family of transmembrane glycoproteins involved in Ca2+-dependent cell to cell interactions. The members of the family are differentially expressed in various tissues and function in the maintenance of tissue integrity, morphogenesis, and migration. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin), neural cadherin (N-cadherin), placental cadherin (P-cadherin) and retinal cadherin (R-cadherin). Type II include kidney cadherin (K-cadherin) and osteoblast cadherin (OB-cadherin). One of the best characterized cadherins is E-cadherin (epithelial cadherin or cadherin-1). It consists of calcium binding extracellular domains responsible for interactions with other E-cadherin molecules on neighboring cells and interaction with integrin alphaEbeta7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently downregulated, which is consistent with its function of an invasion suppressor in normal epithelia.

Immunogène

Whole cells corresponding to human E-Cadherin.

Application

Flow Cytometry Analysis: 2 µg from a representative lot detected E-Cadherin in 1X10E6 A431 cells.

Flow Cytometry Analysis: A representative lot from an independent laboratory detected E-Cadherin in bone marrow cells (Burhing, H. J., et al. (1996). Leukemia. 10(1):106-116.)

Inhibition Analysis: A representative lot from an independent laboratory inhibited E-Cadherin-mediated adhesion of Langerhans to keratinocytes (Caberg, J. H., et al. (2008). Carcinogenesis. 29(7):1441-1447.).

Inhibition Analysis: A representative lot from an independent laboratory inhibits human embryoid body agglomeration (Dang, S. M., et al. (2004). Stem Cells. 22: 275-282.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected E-Cadherin in human thymus tissue (Kutlesa, S., et al. (2002). J Cell Sci. 115(Pt. 23):4505-4515.).

Western Blotting Analysis: A representative lot from an independent laboratory detected E-Cadherin in human thymus tissue lysate (Kutlesa, S., et al. (2002). J Cell Sci. 115(Pt. 23):4505-4515.).

Research Category
Cell Structure

Research Sub Category
ECM Proteins

This Anti-E-Cadherin Antibody, clone 67A4, Azide & Ascites Free is validated for use in western blotting, flow cytometry, inhibition & immunofluorescence for the detection of E-Cadherin.

Qualité

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected E-Cadherin in 10 µg of A431 cell lysate.

Description de la cible

~97 kDa observed

Liaison

Replaces: 04-1103

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable