MAB3199
Anti-E-Cadherin Antibody, clone 67A4
clone 67A4, Chemicon®, from mouse
Synonyme(s) :
CD324
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
67A4, monoclonal
Espèces réactives
human
Fabricant/nom de marque
Chemicon®
Technique(s)
flow cytometry: suitable
western blot: suitable
Isotype
IgG1
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... CDH1(999)
Spécificité
Antibodies against E-cadherin are suitable to characterize epithelial cells. E-Cadherin is involved in adhesion of epithelial cells (mostly homotypic and homophilic). E-cadherin is also expressed on immature erythroid cells. The antibody 67A4 is suitable to study E-cadherin expression by flow cytometry. In addition, it blocks the interaction of E-cadherin molecules and therefore is suitable to study E-cadherin-mediated signal transduction.
Antigen is expressed in non-neural epithelial tissues.
Antigen is expressed in non-neural epithelial tissues.
Immunogène
T-47D breast carcinoma line
Application
Anti-E-Cadherin Antibody, clone 67A4 detects level of E-Cadherin & has been published & validated for use in FC & WB.
Immunoblotting: 123kD in membrane fractions; MCF-7 membranes positive control. Confluent cells were washed three times with ice-cold PBS and lysed in 50mM HEPES pH 7.5, 150mM NaCl, 1mM EDTA, 10% glycerine, 1% triton X-100, 20mM Na(4)P(2)O(7), 2mM Na-vanadate, 100mM NaF, 2mM PMSF, 20μg/mL leupeptin, 1mM pNPP(para-nitrophenylphosphate), 20μg/mL aprotinin, and 200μM NH(4)-molybdate. Crude extracts were clarified by centrifugation (10,000 x g 10 min), and supernatants were taken for immunoprecipitation. Protein concentration of cleared lysates were determined by BCA protein assay. {Buhring, et al Luekemia 10:106-116, 1996}.
FACS Analysis
Functional blocking of E-cadherin
Does not work well for immunoprecipitation
Original paper, Buhring, H et al Leukemia 10:106-116, 1996.
Optimal working dilutions must be determined by end user.
FACS Analysis
Functional blocking of E-cadherin
Does not work well for immunoprecipitation
Original paper, Buhring, H et al Leukemia 10:106-116, 1996.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Liaison
Replaces: 04-1103
Forme physique
Format: Purified
Protein A purified immunoglobulin. Liquid in 0.02M PBS with NaCl, pH=7.6, 0.1% Sodium azide.
Stockage et stabilité
Maintain 2-8°C in undiluted aliquots for up to 12 months after date of receipt.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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