MAB3060
Anti-Paxillin Antibody, clone 349
clone 349, Chemicon®, from mouse
Synonyme(s) :
Anti-AW108311, Anti-AW123232, Anti-Pax
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
349, monoclonal
Espèces réactives
rat, human, mouse, canine, chicken
Fabricant/nom de marque
Chemicon®
Technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
Isotype
IgG1
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... PXN(5829)
rat ... Pxn(360820)
Spécificité
Reacts strongly with two bands on Western blots of total cell lysate from chick embryo fibroblasts or rat brain. The lower molecular band is believed to be either an isoform or a degradation product of the 68 kD protein, since it is found also on SDS-PAGE of affinity-purified paxillin.
Immunogène
Affinity purified phosphotyrosine-containing proteins from Rous sarcoma virus-transformed chick embryo fibroblasts.
Application
Anti-Paxillin Antibody, clone 349 detects level of Paxillin & has been published & validated for use in ELISA, WB, IC, IH.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Western blot: 1:1,000-1:10,000
ELISA
Immunohistochemistry 1:900 in Placenta
Immunocytochemistry
Immunoprecipitation
Optimal working dilutions must be determined by end user.
ELISA
Immunohistochemistry 1:900 in Placenta
Immunocytochemistry
Immunoprecipitation
Optimal working dilutions must be determined by end user.
Liaison
Replaces: 04-581
Forme physique
Format: Purified
Purified immunoglobulin. Liquid in aqueous buffered solution containing 0.09% sodium azide.
Stockage et stabilité
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
Remarque sur l'analyse
Control
POSITIVE CONTROL:
For western blot, load 5-10 μg of whole cell lysate prepared from the human epidermoid carcinoma cell line, A431.
POSITIVE CONTROL:
For western blot, load 5-10 μg of whole cell lysate prepared from the human epidermoid carcinoma cell line, A431.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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M Ishino et al.
FEBS letters, 474(2-3), 179-183 (2000-06-06)
Hic-5 is a CAKbeta-binding protein localized at focal adhesions. Here we show that overexpression of CAKbeta or Fyn, but not FAK, enhanced the tyrosine phosphorylation of coexpressed Hic-5 in COS-7 cells. These phosphorylations were further augmented by stimulating cells with
Role of zyxin in differential cell spreading and proliferation of melanoma cells and melanocytes.
van der Gaag, EJ; Leccia, MT; Dekker, SK; Jalbert, NL; Amodeo, DM; Byers, HR
The Journal of Investigative Dermatology null
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Eph receptors and their corresponding membrane-bound ephrin ligands regulate cell positioning and establish tissue patterns during embryonic and oncogenic development. Emerging evidence suggests that assembly of polymeric Eph signalling clusters relies on cytoskeletal reorganisation and underlies regulation by protein tyrosine
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