MAB079-1
Anti-S-100 Protein Antibody, clone 15E2E2
clone 15E2E2, Chemicon®, from mouse
Synonyme(s) :
Calcium Binding Protein A1/B1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
15E2E2, monoclonal
Espèces réactives
bovine, rat, human, mouse
Fabricant/nom de marque
Chemicon®
Technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotype
IgG2aκ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... S100A1(6271)
Description générale
S-100 protein is a member of the EF-hand calcium-binding protein superfamily that is widely distributed and conserved in the central nervous system of vertebrates. The protein exists in both heterodimeric and homodimeric forms. The two subunits of S-100 protein are the products of separate genes that are differentially expressed by various cells. The beta subunit is present in all S-100 positive cells and tumors. In contrast, the alpha subunit is detectable only in heart, skeletal muscle and brain. In addition to its calcium binding properties, S-100 protein has a high-affinity binding site for zinc and is involved in the regulation of protein phosphorylation in the brain. The protein apparently plays a role in cell differentiation and growth, cytoskeletal structure and function, and has been implicated in neuropathological diseases.
Spécificité
This antibody recognizes ~22 kDa (non-reduced) or 11 kDa (reduced) of S100. It reacts with human, rat, mouse, and cow (bovine). Other species are not tested.
Immunogène
Epitope: Recognizes both alpha & beta S100
Purified bovine S-100 protein
Application
Anti-S-100 Protein Antibody, clone 15E2E2 is an antibody against S-100 Protein for use in IH, IH(P), IP & WB.
Immunoprecipitation:
A previous lot of this antibody was used in IP.
Visualization of cellular and subcellular localization of S-100 protein using immunohistochemical techniques.
Immunohistochemistry:
A 1:100-200 dilution of a previous lot was used for paraffin embedded tissue sections.
Optimal working dilutions must be determined by end user.
A previous lot of this antibody was used in IP.
Visualization of cellular and subcellular localization of S-100 protein using immunohistochemical techniques.
Immunohistochemistry:
A 1:100-200 dilution of a previous lot was used for paraffin embedded tissue sections.
Optimal working dilutions must be determined by end user.
Research Category
Metabolism
Metabolism
Research Sub Category
Muscle Physiology
Muscle Physiology
Qualité
Evaluated by Western Blot on PC12 lysates
Western Blot Analysis:
1:1000 dilution of this antibody detected S100 on 10 µg of PC12 lysate.
Western Blot Analysis:
1:1000 dilution of this antibody detected S100 on 10 µg of PC12 lysate.
Forme physique
Format: Purified
The mouse IgG is purified by protein A-affinity chromatography according to isotyping.
This affinity purified antibody is supplied in sterile phosphate buffered saline (pH 7.2) containing antibody stabilizer. Contains 0.01% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8ºC from date of receipt.
Remarque sur l'analyse
Control
Positive Control Tissue: Melanoma
Positive Control Tissue: Melanoma
Autres remarques
Concentration: 0.5 mg/mL
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Cellular immunolocalization of S100 protein within fixed tissue sections by monoclonal antibodies.
Loeffel, S C, et al.
Archives of Pathology & Laboratory Medicine, 109, 117-122 (1985)
RAGE-TXNIP axis is required for S100B-promoted Schwann cell migration, fibronectin expression and cytokine secretion.
Sbai, O; Devi, TS; Melone, MA; Feron, F; Khrestchatisky, M; Singh, LP; Perrone, L
Journal of Cell Science null
Daria Guseva et al.
Brain : a journal of neurology, 132(Pt 8), 2180-2195 (2009-06-23)
The adhesion molecule L1 is one of the few adhesion molecules known to be beneficial for repair processes in the adult central nervous system of vertebrates by promoting axonal growth and neuronal survival. In the peripheral nervous system, L1 is
Jonathan D Verrier et al.
The Journal of pharmacology and experimental therapeutics, 354(2), 175-183 (2015-05-23)
The 3',5'-cAMP-adenosine pathway (3',5'-cAMP→5'-AMP→adenosine) and the 2',3'-cAMP-adenosine pathway (2',3'-cAMP→2'-AMP/3'-AMP→adenosine) are active in the brain. Oligodendrocytes participate in the brain 2',3'-cAMP-adenosine pathway via their robust expression of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase; converts 2',3'-cAMP to 2'-AMP). Because Schwann cells also express CNPase
Steven Petratos et al.
Journal of neuropathology and experimental neurology, 62(4), 398-411 (2003-05-02)
Schwann cell death is a developmentally regulated phenomenon and is also induced after peripheral nerve axotomy in neonatal rodents. In this study, we explored whether ligand-induced activation of the low-affinity neurotrophin receptor (p75(NTR)) is responsible for inducing Schwann cell death
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