ABT256
Anti-Telo-Collagen Type I, A1/COL1A1
from rabbit, purified by affinity chromatography
Synonyme(s) :
Collagen alpha-1(I) chain, Alpha-1 type I collagen
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
mouse, human, sheep
Technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... COL1A1(1277)
Description générale
Collagen alpha-1(I) chain (UniProt P02452; also known as Alpha-1 type I collagen) is encoded by the COL1A1 gene (Gene ID 1277) in human. Collagen is the major component of the extracellular matrix (ECM) and form the fibrils of tendons, ligaments, and bones. Type I collagen consists of two alpha I chains and one alpha 2 chain. Alpha-1 type I collagen is initially produced as an 1464-amino acid prepro-form with a signal peptide sequence (a.a. 1-22) and two propeptide sequences (a.a. 23-161 and a.a. 1219 –1464), the removal of which yields the mature alpha-1(I) chain. The mature alpha-1(I) chain is composed mostly of a large triple-helical region (a.a. 179-1192) sandwiched between two nonhelical segments known as the N-terminal telopeptide (a.a. 162-178; numbering based on the prepro-form) and the C-terminal telopeptide (a.a. 1193-1218; numbering based on the prepro-form). Collagen can be extracted from tissue via either enzymatic or non-enzymatic means. Collagen extracted using the proteolytic enzyme pepsin corresponds to the large triple-helical region, referred to as atelocollagen because both the N- and C-terminal telopeptides have been cleaved off by pepsin. On the other hand, collagen preparations obtained with non-enzymatic means (e.g. by acid extraction) have the intact telopeptides at both ends.
Spécificité
Cat. No. ABT256 Anti-Telo-Collagen Type I, A1/COL1A1 was raised against a sequence derived from the C-terminal telopeptide (not the N-terminal telopeptide) region. This polyclonal antibody will detect prepro-, pro-, and mature collagen alpha-1(I) chain, but not atelocollagen prepared with proteolytic enzymes due to the absence of the C-terminal telopeptide sequence.
Predicted to react with a broad range of species based on high sequence homology, including mammalian, avian, and amphibian species.
Immunogène
Epitope: C-terminal telopeptide region
Linear peptide corresponding to sequence derived from human Collagen Type I, A1/COL1A1 C-terminal telopeptide region.
Application
Anti-Telo-Collagen Type I Antibody, A1/COL1A1 is an antibody against Telo-Collagen Type I for use in Western Blotting, Flow Cytometry, Immunohistochemistry.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Western Blotting Analysis: A representative lot detected Telo-Collagen Type I, A1/COL1A1 in mouse lung and skin tissue lysate.
Flow Cytometry Analysis: A representative lot detected Telo-Collagen Type I, A1/COL1A1 in mouse lung tissue.
Immunohistochemistry Analysis: A representative lot detected Telo-Collagen Type I, A1/COL1A1 in mouse lung tissue.
Flow Cytometry Analysis: A representative lot detected Telo-Collagen Type I, A1/COL1A1 in mouse lung tissue.
Immunohistochemistry Analysis: A representative lot detected Telo-Collagen Type I, A1/COL1A1 in mouse lung tissue.
Qualité
Evaluated by Western Blotting in mouse liver tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Telo-Collagen Type I, A1/COL1A1 in 1 µg of mouse liver tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Telo-Collagen Type I, A1/COL1A1 in 1 µg of mouse liver tissue lysate.
Description de la cible
~180 kDa observed
Forme physique
Affinity purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
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