ABT1383

Anti-phospho-Keratin-18 Antibody (Ser52)

from rabbit, purified by affinity chromatography

• Synonyme(s) : Keratin, type I cytoskeletal 18, Ser52-phosphorylated, Cell proliferation-inducing gene 46 protein, Ser52-phosphorylated, CK-18, Ser52-phosphorylated, Cytokeratin-18, Ser52-phosphorylated, K18, Ser52-phosphorylated, Keratin-18, Ser52-phosphorylated
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Technique(s): western blot: suitable
• Numéro d'accès NCBI: NP_000215
• Numéro d'accès UniProt: P05783
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: phosphorylation (pSer52)
• Informations sur le gène: human ... KRT18(3875)

Description

Description générale

Keratin, type I cytoskeletal 18 (UniProt P05783; also known as Cell proliferation-inducing gene 46 protein, CK-18, Cytokeratin-18, K18, Keratin-18) is encoded by the KRT18 (also known as CYK18, PIG46) gene (Gene ID 3875) in human. Cytokeratin-18 (Keratin-18 or K18) belongs to a family of highly conserved epithelial-specific intermediate filament (IF) proteins that play an important role in the mechanical stability and integrity of epithelial cells and tissues. Each epithelium is characterized by its unique keratin expression pattern. All epithelial cells typically express at least one type-I and one type-II keratin, which form noncovalent heteropolymers. Keratin 18 is a type I intermediate filament protein that pairs with keratin 8 (K8/K18) in simple epithelia and plays an important role in protecting cells from stress, injury, and apoptosis. K18 is the only type-I keratin in adult hepatocytes and the major type-I keratin in many colonic tumor cell lines.The structure and function of K8/18 are regulated by posttranslational modifications, such as glycosylation, ubiquitination, and phosphorylation, including K8 phosphorylation on Ser8, Ser23, Ser73, Ser431, and K18 phosphorylation on Ser33 and Ser52. K18 phosphorylation has been proposed as a progression marker for human chronic hepatitis B (CHB). Progressively higher Ser52 phosphorylation is reported in non-cirrhotic and cirrhotic CHB livers, and progressive increase of Ser33 and Ser52 phosphorylation correlates with the elevation of both histological lesions and enzymatic activities of alanine aminotransferase in non-cirrhotic CHB livers.

Spécificité

Target phosphorylation site is not conserved in murine and rat species.

This rabbit polyclonal antibody detected upregulated cellular Keratin-18 Ser52 phosphorylation upon phosphatase inhibitors treatment. The Ser52 numbering is based on keratin-18 sequence without the initiation Met1, which was removed posttranslationally.

Immunogène

KLH-conjugated linear peptide corresponding to human Keratin-18 target region sequence containing phosphorylated Ser52.

Application

This rabbit polyclonal Anti-phospho-Keratin-18 (Ser52) Antibody, Cat. No. ABT1383 is validated for use in Western Blotting for the detection of Keratin-18 Ser52 phosphorylation.

Qualité

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected upregulated Keratin-18 Ser52 phosphorylation in 10 µg lysate from phosphatase inhibitors Calyculin A- and Okadaic acid-treated HEK293 cells.

Description de la cible

~48 kDa observed. 47.93 kDa (Met1 removed) calculated. Uncharacterized bands may be observed in some lysate(s).

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable