ABS574

Anti-Surfactant Protein D

from rabbit, purified by affinity chromatography

• Synonyme(s) : Pulmonary surfactant-associated protein D, Collectin-7, Lung surfactant protein D, PSP-D, SP-D
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Technique(s): immunohistochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_003010
• Numéro d'accès UniProt: P35247
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

Pulmonary surfactant-associated protein D (UniProt P35247; also known as Collectin-7, Lung surfactant protein D, PSP-D, SP-D) is encoded by the SFTPD (also known as COLEC7, PSPD, SFTP4) gene (Gene ID 6441) in human. Surfactant, produced by alveolar type II cells and airway Clara cells, resides at the air-liquid interface of the lungs and is most commonly known for its role in reducing surface tension. Surfactant is a lipoprotein complex composed of approximately 10% proteins, including surfactant protein A (SP-A), SP-B, SP-C, and SP-D. SP-A and SP-D belong to the collectin family of proteins and can modulate innate immunity. SP-D is known to enhance pulmonary clearance of pathogens, such as Pseudomonous aerginosa, Klebsiella pneumonia, respiratory syncytial virus (RSV) and Influenza virus. SP-D is also shown to modify allergic responsee in the lungs by binding common allergens, including house dust mite and pollen particles. SP-A-deficient and SP-D-deficient mice are susceptible to bleomycin-induced lung injury and display increased cellular inflammation, more severe lung fibrosis, and reduced survival. In addition to lung, SP-D is also reported to be detected in extrapulmonary tissues, such as kidney and pancreas. Serum levels of either SP-A or SP-D in human are correlated with mortality in patients with pulmonary fibrosis. Human SP-D is produced with a signal peptide sequence (a.a. 1-20), which is removed posttranslationally to yield the mature protein (a.a. 21-375) that contains a collagen-like domain (a.a. 46-222), a coiled coil region (a.a. 223-252), and a C-type lectin domain (a.a. 260-375).

Spécificité

This rabbit polyclonal antibody targets a sequence within the C-type lectin domain of human surfactant protein D.

Immunogène

Epitope: domain of:

KLH-conjugated linear peptide corresponding to a sequence within the C-type lectin domain of human surfactant protein D.

Application

Detect Surfactant Protein D using this rabbit polyclonal Anti-Surfactant Protein D Antibody, Cat. No. ABS574, validated for use in Immunohistochemistry and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected surfactant protein D in human lung and pancreas tissue sections.

Research Category
Signaling

Qualité

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected surfactant protein D in 10 µg of A549 cell lysate.

Description de la cible

~48 kDa observed. Target band size appears larger than the calculated molecular weights of 35.50/37.73 kDa (mature/pro-form) due to posttranslational modifications. Uncharacterized bands may be observed in some lysate(s).

Liaison

Replaces: AB3434

Forme physique

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable