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ABS552

Sigma-Aldrich

Anti-p40 Antibody

from rabbit, purified by affinity chromatography

Synonyme(s) :

Tumor protein 63, p63, Chronic ulcerative stomatitis protein, CUSP, Keratinocyte transcription factor KET, Transformation-related protein 63, TP63, Tumor protein p73-like, p73L, p40, p51

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100
300

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

human

Réactivité de l'espèce (prédite par homologie)

mouse (based on 100% sequence homology)

Technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TP63(8626)

Description générale

p40, also known as Tumor protein 63, p63, Chronic ulcerative stomatitis protein, CUSP, Keratinocyte transcription factor , KET, Transformation-related protein 63, TP63, Tumor protein p73-like, p73L, p40, or p51, and encoded by the gene TP63/KET/P63/P73H/P73L/TP73L, is a member of the p53 protein family. p40 is a sequence specific DNA binding transcriptional activator or repressor depending upon the target gene and the isoform involved. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specificactivity. p40 is involved in Notch signaling and plays a role in p53/TP53 dependent apoptosis and epithelial morphogenesis. Moreover p40, developmentally, is required for proper limb formation. p40 is expressed widely, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues. Mutations in p40 lead to a variety of dysplasias and malformations, including Acro-dermato-ungual-lacrimal-tooth syndrome (ADULT syndrome), Ankyloblepharon-ectodermal defects cleft lip/palate disorder (AEC), Ectrodactyly, ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3), Split-hand/foot malformation 4 (SHFM4), Limb-mammary syndrome (LMS), Ectodermal dysplasia, Rapp-Hodgkin type (EDRH), Non-syndromic orofacial cleft 8 (OFC8).

Spécificité

Uniprot describes multiple isoforms for this protein and based on the immunogen this antibody should recognize isoforms 2,4,6,8,10 and 12.

Immunogène

Epitope: Transcription activation domain
KLH-conjugated linear peptide corresponding to the transcription activation domain of human p40.

Application

Anti-p40 | ABS552 is an antibody against p40 for use in Western Blotting, Immunohistochemistry (Paraffin).
Research Category
Signaling
Research Sub Category
Signaling Neuroscience
Western Blotting Analysis: 2.0 µg/mL from a representative lot detected p40 in 10 µg of human liver and human skin tissue lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Isocitrate dehydrogenase in squamous carcinoma cells.

Qualité

Evaluated by Western Blotting in human heart tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected p40 in 10 µg of human heart tissue lysate.

Description de la cible

~63/56/51/46 kDa observed. Uncharacterized band(s) may appear in some lysates.

Forme physique

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Jacob J Tokar et al.
SLAS technology, 22(3), 348-357 (2017-03-17)
Although average survival rates for lung cancer have improved, earlier and better diagnosis remains a priority. One promising approach to assisting earlier and safer diagnosis of lung lesions is bronchoalveolar lavage (BAL), which provides a sample of lung tissue as
Minmin Song et al.
eLife, 10 (2021-06-17)
Emerging evidence demonstrates the important role of circular RNAs (circRNAs) in regulating pathological processes in various diseases including organ fibrosis. Endometrium fibrosis is the leading cause of uterine infertility, but the role of circRNAs in its pathogenesis is largely unknown.
Theresa Casey et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 311(6), R1125-R1134 (2016-10-07)
Circadian clocks influence virtually all physiological processes, including lactation. Here, we investigate the role of the CLOCK gene in regulation of mammary epithelial cell growth and differentiation. Comparison of mammary morphology in late-pregnant wild-type and ClockΔ19 mice, showed that gland
Kuijie Liu et al.
Oncotarget, 8(17), 29101-29115 (2017-04-19)
Among the diverse co-regulatory relationships between transcription factors (TFs) and microRNAs (miRNAs), feedback loops have received the most extensive research attention. The co-regulation of TFs and miRNAs plays an important role in colorectal cancer (CRC) growth. Here, we show that
Jutaro Fukumoto et al.
American journal of physiology. Cell physiology, 316(4), C492-C508 (2019-01-17)
Aging is a key contributor for subclinical progression of late-onset lung diseases. Basal, club, and type II alveolar epithelial cells (AECs) are lung epithelial progenitors whose capacities of differentiation are extensively studied. The timely transition of these cells in response

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