ABN2192
Anti-Calbindin D-28K
from rabbit
Synonyme(s) :
Calbindin, Calbindin D28, D-28K, PCD-29, Spot 35 protein, Vitamin D-dependent calcium-binding protein
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
mouse, human, rat
Conditionnement
antibody small pack of 25 μL
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... CALB1(793)
mouse ... Calb1(12307)
Description générale
Calbindin (UniProt: P12658; also known as Calbindin D28, D-28K, PCD-29, Spot 35 protein, Vitamin D-dependent calcium-binding protein, avian-type) is encoded by the calb1 gene (gene ID: 12307) in murine species. Calbindin D-28k is a member of a large family of intracellular calcium-binding proteins of the EF-hand related to calmodulin and troponin-C. Calbindin D-28K has four functional calcium-binding sites (aa 24-35; 111-122; 155-166; and 199-210). It has moderate cytoplasmic mobility and comprises six EF-hands, of which one or two are non-functioning in metal binding. EF-2 is viewed as non-metal binding and EF-6 may have a very low Ca2+ affinity. Four EF hands function in calcium binding with rapid to intermediate kinetics and affinity. The biological roles of Calbindin D-28K include calcium regulation and signaling, including calcium transport and uptake, calcification of bone and teeth, and calcium related signaling in neurons and transiently in embryological development. It also protects neurons from apoptotic cell death. Decline in Calbindin D-28K levels in hippocampal dentate granule cells correlates with the kindling model for epilepsy. Calbindin D-28K immunoreactivity is shown to be specifically absent from neuronal populations lost early in ALS, while substantial loss of neurons, which do contain this protein occur in patients with Huntington′s disease. (Ref.: Schmidt, H (2012). Front. Mol. Neurosci. 5; 25).
Spécificité
This rabbit polyclonal antibody detects Calbindin D-28K in Human, Murine, and Rat cells. It targets an epitope within 107 amino acids from the C-terminal region.
Immunogène
Epitope: C-terminus
GST/His-tagged recombinant fragment corresponding to 107 amino acids from the C-terminal end of murine Calbindin D-28K.
Application
Anti-Calbindin D-28K Antibody, Cat. No. ABN2192, is a highly specific rabbit polyclonal antibody that targets Calbindin D and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Calbindin D-28K in E18 rat cortical cells.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Calbindin D-28K in mouse kidney and human cerebellum tissue sections.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Calbindin D-28K in mouse kidney and human cerebellum tissue sections.
Research Category
Neuroscience
Neuroscience
Qualité
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Calbindin D-28K in human brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Calbindin D-28K in human brain tissue lysate.
Description de la cible
~28 kDa observed; 29.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Forme physique
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Hyun Seung Shin et al.
International journal of molecular sciences, 24(5) (2023-03-12)
Early life stress (ELS) in developing children has been linked to physical and psychological sequelae in adulthood. In the present study, we investigated the effects of ELS on brain and behavioral development by establishing a novel ELS model that combined
Yuxuan Meng et al.
Methods in molecular biology (Clifton, N.J.), 2800, 67-74 (2024-05-06)
The study of cell signaling within tissues can be enhanced using highly multiplexed immunohistochemistry to localize the presence and spatial distribution of numerous pathways of interest simultaneously. Additional data can also be gained by placing the identified proteins into the
Sahithi Attaluri et al.
Frontiers in aging neuroscience, 15, 1200445-1200445 (2023-07-10)
Extracellular vesicles (EVs) released by human-induced pluripotent stem cell (hiPSC)-derived neural stem cells (NSCs) have robust antiinflammatory and neurogenic properties due to therapeutic miRNAs and proteins in their cargo. Hence, hiPSC-NSC-EVs are potentially an excellent biologic for treating neurodegenerative disorders
Song Li et al.
Cell reports, 41(13), 111880-111880 (2022-12-29)
Aging causes an irreversible, cumulative decline in neuronal function. Using the visual system as a model, we show that astrocytes play a critical role in maintaining retinal ganglion cell health and that deletion of SPP1 (secreted phosphoprotein 1, or osteopontin)
Abeer Dagra et al.
NPJ Parkinson's disease, 7(1), 76-76 (2021-08-20)
Pathophysiological damages and loss of function of dopamine neurons precede their demise and contribute to the early phases of Parkinson's disease. The presence of aberrant intracellular pathological inclusions of the protein α-synuclein within ventral midbrain dopaminergic neurons is one of
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