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539131

Sigma-Aldrich

Protease Inhibitor Cocktail I

lyophilized, for the inhibition of aspartic, cysteine, serine, metalloproteases and aminopeptidases

Synonyme(s) :

Protease Inhibitor Mix, Protease inhibitor

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.28

product name

Protease Inhibitor Cocktail Set I, A cocktail of five protease inhibitors that will inhibit a broad range of proteases and esterases. Supplied with a data sheet.

Forme

lyophilized

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
desiccated (hygroscopic)

Conditions d'expédition

wet ice

Température de stockage

−20°C

Catégories apparentées

Description générale

Protease Inhibitor Cocktail Set I A is specially formulated containing five protease inhibitors that will inhibit a broad range of proteases and esterases.

Spécificité

Inhibits a broad range of proteases and esterases.

Application

Protease Inhibitor Cocktail Set I – Calbiochem has been used in lysis buffer to lyse cells and perform western blotting.
Protease Inhibitor Cocktail Set I have been used as a supplement in:
  • cold radioimmunoprecipitation assay (RIPA) buffer to prepare cell lysates for western blotting
  • RIPA lysis buffer to extract total protein from rat liver tissues and AML12 cells
  • sucrose solution to homogenize mice cortices for myelin isolation

Actions biochimiques/physiologiques

Protease inhibitor cocktails and specific protease inhibitors are essential tools utilized by scientists in various proteomic studies. These studies encompass protein expression, characterization, biomarker discovery, mapping post-translational modifications, protein expression profiling, and quantitative protein measurement. The application of a protease inhibitor cocktail or protease inhibitors guarantees the preservation of protein integrity, facilitating downstream analysis and characterization.

Caractéristiques et avantages

  • Protease Inhibitor Cocktails offer protection of target protein against degradation in crude extracts
  • Ready for reconstitution

Avertissement

Toxicity: Harmful (C)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Reconstitute each vial with 1 ml dH2O to yield a 100X stock solution.

Autres remarques

The presence of EDTA may interfere with the purification of His•Tag fusion proteins if a cell extract is applied directly to IMAC resins.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Health hazardCorrosionExclamation mark
GHS08,GHS05,GHS07

Mention d'avertissement

Danger

Mentions de danger

H314,H332,H373

Classification des risques

Acute Tox. 4 Inhalation - Eye Dam. 1 - Skin Corr. 1A - STOT RE 2 Inhalation

Organes cibles

Respiratory Tract

Code de la classe de stockage

8B - Non-combustible corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Tamta Arakhamia et al.
Cell, 180(4), 633-644 (2020-02-08)
Tau aggregation into insoluble filaments is the defining pathological hallmark of tauopathies. However, it is not known what controls the formation and templated seeding of strain-specific structures associated with individual tauopathies. Here, we use cryo-electron microscopy (cryo-EM) to determine the
Jeffrey W Ecker et al.
Vaccines, 8(3) (2020-08-23)
Influenza viruses infect millions of people each year, resulting in significant morbidity and mortality in the human population. Therefore, generation of a universal influenza virus vaccine is an urgent need and would greatly benefit public health. Recombinant protein technology is
Neuronal deletion of Gtf2i results in developmental microglial alterations in a mouse model related to Williams syndrome
Ela B, et al.
Glia, 72, 1117-1135 (2024)
Arginase-1 inhibition reduces migration ability and metastatic colonization of colon cancer cells
Wang X, et al.
Cancer & Metabolism, 11(1), 1-1 (2023)
Huasheng Liang
Oncology letters, 19(4), 3215-3222 (2020-03-29)
The aim of the present study was to determine the mechanism by which advanced glycation end products (AGEs) induce proliferation, invasion and epithelial-mesenchymal transition (EMT) of human colon cancer SW480 cells. SW480 cells were divided into groups as follows: i)
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