374825
Hemocyanin, Keyhole Limpet, Megathura crenulata, High Purity, Endotoxin-Free, Sterile-Liquid
Hemocyanin, Keyhole Limpet, Megathura crenulata, High Purity, Endotoxin Free, is a large, multi-subunit, O2-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.
Synonyme(s) :
Hemocyanin, Keyhole Limpet, Megathura crenulata, High Purity, Endotoxin-Free, Sterile-Liquid
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About This Item
Produits recommandés
Niveau de qualité
Pureté
≥95% (size exclusion chromatography)
Forme
liquid
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
do not freeze
Impuretés
≤25 EU/mg Endotoxin
≥1.5 x 10⁻³ copper to protein ratio
≥5 mg/mL Protein
Couleur
clear bluish
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Description générale
Keyhole limpet hemocyanin (KLH) is a large, multi-subunit, O2-carrying, metalloprotein. This high molecular weight glycoprotein is extracted from a marine snail species called Megathura crenulate.
Application
Hemocyanin, Keyhole Limpet, Megathura crenulata, high purity, endotoxin-free, sterile-liquid has been used as an antigen to test the polyreactivity of monoclonal antibody (mAb) using enzyme-linked immunosorbent assay(ELISA). It has also been used to immunize animals to study KLH-specific ex vivo recall responses and specific T-cell dependent antibody response (TDAR) in monkeys.
Actions biochimiques/physiologiques
Keyhole limpet hemocyanin (KLH) stimulates both cell-mediated and humoral responses in animals and humans. It elicits immunostimulatory properties and also functions as a vaccine adjuvant. KLH serves as a carrier protein to produce antibodies and haptens like peptides and oligosaccharides. It is used to treat bladder cancer.
Conditionnement
Please refer to vial label for lot-specific concentration.
Avertissement
Toxicity: Standard Handling (A)
Forme physique
In 83 mM sodium phosphate, 150 mM NaCl, pH 7.2.
Notes préparatoires
KLH is an extremely large protein complex that has a tendency to aggregate and precipitate from solutions. Use a buffer when diluting KLH and maintain the magnesium concentration at ≥10 mM. Store KLH solutions at 4°C. DO NOT freeze, vortex or agitate to dissolve KLH. Freezing or agitating KLH will cause protein aggregation and precipitation.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Oyindasola Oyelaran et al.
Proteomics. Clinical applications, 4(3), 285-294 (2010-12-08)
Keyhole limpet hemocyanin (KLH) is used as a vaccine adjuvant, as a carrier protein for small haptens, and as a treatment for bladder cancer. Immunization with KLH produces antibodies to tumor-associated carbohydrate antigens (TACAs) in animals, and these antibodies have
Joshua T Gamse et al.
Toxicology and applied pharmacology, 409, 115285-115285 (2020-10-19)
The OX40 receptor plays a crucial co-stimulatory role in T effector cell survival, expansion, cytokine production, and cytotoxicity to tumor cells; therefore, OX40 agonists are being evaluated as anti-cancer immunotherapies, especially in combination with checkpoint inhibitors. To support clinical development
Erik H J G Aarntzen et al.
Cancer immunology, immunotherapy : CII, 61(11), 2003-2011 (2012-04-25)
Keyhole limpet hemocyanin (KLH) attracts biomedical interest because of its remarkable immunostimulatory properties. Currently, KLH is used as vaccine adjuvant, carrier protein for haptens and as local treatment for bladder cancer. Since a quantitative human anti-KLH assay is lacking, it
Jenna J Guthmiller et al.
Immunity, 53(6), 1230-1244 (2020-10-24)
Polyreactivity is the ability of a single antibody to bind to multiple molecularly distinct antigens and is a common feature of antibodies induced upon pathogen exposure. However, little is known about the role of polyreactivity during anti-influenza virus antibody responses.
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