219466
CELLULYSIN® Cellulase, Trichoderma viride
Synonyme(s) :
β-D-glucosidase, endo-β-1,4 glucanase, cellobiohydrolase
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About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.54
Produits recommandés
Source biologique
fungus (Trichoderma viride)
Forme
solid
Activité spécifique
≥10,000 units/g dry wt.
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
OK to freeze
Technique(s)
transformation: suitable
Couleur
yellow-white
Solubilité
water: soluble
Adéquation
suitable for molecular biology
Application(s)
food and beverages
pharmaceutical
Conditions d'expédition
ambient
Température de stockage
2-8°C
Description générale
Cellulase includes endo-β-1,4 glucanase, cellobiohydrolase, and β-D-glucosidase. It is commonly produced by bacteria and fungi.
Application
CELLULYSIN® Cellulase, Trichoderma viride has been used:
- to digest transgenic BY-2 cells exposed to paraformaldehyde fixation
- in the enzyme mix used for in situ hybridization to plant chromosomes
- in the enzyme solution for the transformation of Phytophthora species
- to isolate intact cells and protoplasts from plant tissues
Actions biochimiques/physiologiques
Cellulase is useful in food, feed, textile, leather, detergent, and paper industries. It is also applied in fiber modification, biomass fermentation, and pharmaceutical industries.
Avertissement
Toxicity: Harmful (C)
Définition de l'unité
One unit is a measure of decomposing activity using a filter paper substrate at 40°C, pH 4.0.
Reconstitution
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 days at 4°C or for up to 1 month at -20°C.
Autres remarques
Takebe, I., et al. 1968. Plant Cell Physiol.9, 115.
Tomita, Y., et al. 1968. J. Ferment. Technol.46, 701.
Tomita, Y., et al. 1968. J. Ferment. Technol.46, 701.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
CELLULYSIN is a registered trademark of Merck KGaA, Darmstadt, Germany
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Les clients ont également consulté
Weizhen Wang et al.
Frontiers in microbiology, 10, 2402-2402 (2019-11-12)
Phytophthora, a genus of oomycetes, contains many devastating plant pathogens, which cause substantial economic losses worldwide. Recently, CRISPR/Cas9-based genome editing tool was introduced into Phytophthora to delineate the functionality of individual genes. The available selection markers for Phytophthora transformation, however
Farjana Islam et al.
BMC research notes, 11(1), 445-445 (2018-07-06)
This study was conducted to isolate, screening and purification of cellulase from bacteria present in sugar industry waste (molasses) and characterization by morphological and biochemical analysis. Based on experiments, three bacterial strains produced clear transparent zone into carboxymethyl cellulose (CMC)
In Situ Hybridization to Plant Chromosomes
Fluorescence In Situ Hybridization (FISH), 447-494 null
Man-Wah Li et al.
PloS one, 10(8), e0135033-e0135033 (2015-08-14)
Arabidopsis High Mobility Group Box (HMBG) proteins were previously found associated with the interphase chromatin but not the metaphase chromosome. However, these studies are usually based on immunolocalization analysis involving paraformaldehyde fixation. Paraformaldehyde fixation has been widely adapted to preserved
Rahman Ebrahimzadegan et al.
Scientific reports, 9(1), 19989-19989 (2019-12-29)
Here, we characterized the basic properties of repetitive sequences in essential A and supernumerary B chromosomes of Festuca pratensis Huds. This was performed by comparative analysis of low-pass Illumina sequence reads of B chromosome lacking (-B) and B chromosome containing
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