1.15943

Methylene blue (C.I.52015)

for microscopy Certistain^®

• Synonyme(s) : Methylene Blue trihydrate, Methylthioninium chloride, 3,7-Bis(dimethylamino)phenothiazinium chloride, Tetramethylthionine chloride
• Formule empirique (notation de Hill): C₁₆H₁₈ClN₃S · 3H₂O
• Numéro CAS: 7220-79-3
• Poids moléculaire : 373.90
• Numéro Beilstein : 3599847
• Numéro MDL: MFCD00012111
• Code UNSPSC : 41116130
• Numéro de classement CE : 200-515-2

Propriétés

• Niveau de qualité: 400
• Forme: solid
• Puissance: 1180 mg/kg LD₅₀, oral (Rat)
• Composition: Dye, ≥82% spectrophotometric assay
• pH: 3 (20 °C, 10 g/L in H₂O)
• Pf: 190 °C (dec.) (lit.)
• Solubilité: 50 g/L
• Densité: 1.757 g/cm³ at 22 °C
• Masse volumique apparente: 400‑600 kg/m³
• εmax: 2250-2750 at 660-665 nm in 50% ethanol at 0.003 g/L
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: 2-30°C
• Chaîne SMILES: [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
• Clé InChI: CXKWCBBOMKCUKX-UHFFFAOYSA-M

Description

Description générale

Methylene blue (C.I. 52015) for microscopy Certistain^® is a dry dye that is used in bacteriology for an overview stain and as counterstaining for Ziehl-Neelsen method in sample material of human origin. Bacteria appear blue. The dye has a further important application in hematology: Methylene blue and eosin are the main components of many staining solutions and dye mixtures. Certistain^® is the product group for seleceted high quality dyes that fulfill all quality requirements of IVD and CE registration. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Remarque sur l'analyse

Dye content (spectrophotometrically): ≥ 82 %
Identity
- Chloride:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (ethanol 50 %): 660 - 665 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; ethanol 50 %): 2250 - 2750
TLC-Test: passes test
Loss on drying (110 °C): 10 - 15 %
Suitability for microscopy: passes test

Informations légales

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H302
• Conseils de prudence: P264 - P270 - P301 + P312 - P501
• Classification des risques: Acute Tox. 4 Oral
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable