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1.11699

Millipore

Differential reinforced clostridial broth (DRCM)

GranuCult® plus, suitable for microbiology, for Clostridium spp.

Synonyme(s) :

Clostridium differential broth, DRCM

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About This Item

Code UNSPSC :
41106213
Nomenclature NACRES :
NA.74

Agence

DIN Norm 38411

Niveau de qualité

200

Stérilité

non-sterile

Forme

granular

Conditionnement

pkg of 500 g

Fabricant/nom de marque

GranuCult® plus

Conditions de stockage

dry media (Keep tightly closed)

Technique(s)

microbiological culture: suitable

Couleur

brown

pH

7.0-7.2 (25 °C, 28 g/L in H2O, after autoclaving)

Solubilité

27.5 g/L

Masse volumique apparente

650 kg/m3

Application(s)

food and beverages
microbiology

Température de stockage

15-25°C

Adéquation

Clostridium spp.

Description générale

Differential Reinforced Clostridial Broth is used to determine the count of sulfite-reducing bacteria by the MPN technique. The redox indicator resazurin is used to monitor anaerobiosis. Clostridia reduce sulfite to sulfide, which causes the culture medium to turn black. As other bacteria can also produce sulfide, vegetative forms must first be removed from the culture by a relevant treatment (e.g., pasteurization), and the anaerobic spore-forming microorganisms must then be identified. The addition of polymyxin (70 IU/ml) to the broth inhibits the growth of most non-spore-forming microorganisms.

Application

Differential reinforced clostridial broth is suitable for the enumeration of all clostridia by the MPN method in foodstuffs and other materials.

Caractéristiques et avantages

  • GranuCult® offers superior granulated culture media
  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
  • Excellent wettability, solubility, and free-flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions
  • High batch-to-batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allows many supplements to be included, with no need to add these separately

Remarque sur l'analyse

Appearance (clearness): clear
Appearance (colour): reddish-brown
pH-value (25 °C): 6.9 - 7.3
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Bacillus cereus ATCC 11778 (WDCM 00001)): fair to good
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): poor to fair
Growth (Clostridium bifermentans ATCC 19299): good to very good
Growth (Clostridium perfringens ATCC 13124 (WDCM 00007)): good to very good
Growth (Clostridium perfringens ATCC 10543): good to very good
Growth (Clostridium sporogenes ATCC 11437): good to very good
Growth (Clostridium sporogenes ATCC 19404 (WDCM 00008)): good to very good
Blacking (Escherichia coli ATCC 25922 (WDCM 00013)): -
Blacking (Bacillus cereus ATCC 11778 (WDCM 00001)): -
Blacking (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): -
Blacking (Clostridium bifermentans ATCC 19299): +
Blacking (Clostridium perfringens ATCC 13124 (WDCM 00007)): +
Blacking (Clostridium perfringens ATCC 10543): +
Blacking (Clostridium sporogenes ATCC 11437): +
Blacking (Clostridium sporogenes ATCC 19404 (WDCM 00008)): +
Incubation: 7 days; 30 °C; layered with paraffin.

Note de bas de page

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informations légales

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

1.07160

Paraffin, viscous Reag. Ph Eur

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H319

Classification des risques

Eye Irrit. 2

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

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