1.09270

Papanicolaou′s solution 3c Polychromatic solution EA 65

for cytology

• Code UNSPSC : 41116113
• Nomenclature NACRES : NA.77

Propriétés

• Niveau de qualité: 400
• Forme: liquid
• IVD: for in vitro diagnostic use
• pH: 5.8 (20 °C in H₂O)
• Température de transition: flash point 15 °C
• Densité: 0.82 g/cm³ at 20 °C
• Application(s): clinical testing; diagnostic assay manufacturing; hematology; histology
• Température de stockage: 15-25°C

Description

Description générale

Papanicolaou′s solution 3c polychromatic solution EA 65 - for cytology, is a ready-to-use solution used for human-medical cell diagnosis and serve the purpose of the cytological investigation of sample material of human origin, for example, gynecological and clinico-cytological specimen materials. Papanicolaou′s technique is the most used staining procedure for cytological specimen is and is intended for the the staining of exfoliative cells in cytological specimens. In the first step, the cell nuclei are stained either progressively or regressively with a hematoxylin solution, (Papanicolaou′s solution 1a Harris hematoxylin solution, (Product number 1.09253) and Papanicolaou′s solution 1b Hematoxylin solution S, (Product number 1.09254)). Nuclei are stained blue to dark violet. In the progressive hematoxylin staining method, staining is carried out to the endpoint, after which the slide is blued in tapwater. With the regressive method the material is over-stained and the excess of staining solution is removed by acid rinsing steps, followed by the bluing step. The structures of nuclei are more differentiated and better visible by the regressive method. The second staining step is cytoplasmic staining by orange staining solution (Papanicolaou′s solution 2b Orange II solution, Product number 1.106887 or Papanicolaou′s solution 2a Orange G solution (OG6), Product number 1.06888), especially for demonstration of mature and keratinized cells. The target structures are stained orange in different intensities. In the third staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown, (Papanicolaou′s solution 3a polychromatic solution EA 31, (Product number 1.09271), Papanicolaou′s solution 3b polychromatic solution EA 50, (Product number 1.09272), Papanicolaou′s solution 3c polychromatic solution EA 65 (Product number 1.09270), o Papanicolaou′s solution 3d polychromatic solution EA 65, (Product number 1.09269). The polychrome solution is used for demonstration of differentiation of squamous cells. The Papanicolaou′s solution 3c polychromatic solution EA 65 will result in a staining with a light-brown to red staining result.
The 100 ml bottle provides 300 - 400 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Application

Cytoplasm staining esp. for non-gynecological specimen, color effect light red

Remarque sur l'analyse

Suitability for microscopy (Vaginal smear)passes testNucleiblue to dark violetCytoplasmred

Informations sur la sécurité

• Pictogrammes: GHS02,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H225 - H317 - H319
• Conseils de prudence: P210 - P233 - P240 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Flam. Liq. 2 - Skin Sens. 1
• Code de la classe de stockage: 3 - Flammable liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): 59.0 °F
• Point d'éclair (°C): 15 °C