05-1923

Anti-phospho-Threonine Antibody, clone 20H6.1

clone 20H6.1, from mouse

• Synonyme(s) : Threonine
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Type de produit anticorps: primary antibodies
• Clone: 20H6.1, monoclonal
• Espèces réactives: mouse, human
• Réactivité de l'espèce (prédite par homologie): vertebrates (based on 100% sequence homology), all
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Isotype: IgG2bκ
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: phosphorylation (pThr)

Description

Description générale

Protein phosphorylation is normally associated with protein modification in signaling pathways and may be useful in pathophysiology and treatment of major neuropsychiatric disorders. Antibodies against phospho-threonine have been used to establish stability of the phosphorylated forms of proteins that may be involved in many different types of signaling. Phospho-threonine antibody is used to measure phosphorylation levels of PSII reaction center proteins D1, D3 and chlorophyll a binding protein CP43.

Immunogène

KLH-conjugated linear peptide corresponding to Threonine.

Application

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Threonine from 100 µg of A431 cells stimulated with calyculin/okadaic acid.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Threonine in A431, NIH/3T3, and HeLa cells.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

This phospho-Threonine antibody is validated for use in WB & ICC for the detection of the phospho-Threonine protein.

Qualité

Evaluated by Western Blot in calyculin/okadaic acid treated and untreated A431 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Threonine on 10 µg of calyculin/okadaic acid treated and untreated A431 cell lysates.

Description de la cible

Various observed

Forme physique

Format: Purified

Protein G

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Calyculin/okadaic acid treated and untreated A431 cell lysates.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable