04-642
Anti-Ago2 Antibody, clone 9E8.2
ascites fluid, clone 9E8.2, Upstate®
Synonyme(s) :
Argonaute-2, Eukaryotic translation initiation factor 2C, AGO2, eIF-2C, Slicer protein, MGC3183, Piwi domain protein, PPD
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
ascites fluid
Type de produit anticorps
primary antibodies
Clone
9E8.2, monoclonal
Espèces réactives
human
Fabricant/nom de marque
Upstate®
Technique(s)
ChIP: suitable (ChIP-seq)
western blot: suitable
Isotype
IgG1κ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... AGO2(27161)
Description générale
Ago2 (argonaute-2), also known as eukaryotic translation initiation factor 2C (EIF2C2), is an essential component for siRNA-directed RNA interference (RNAi) response. Ago2 is an endonuclease required for the unwinding of siRNA duplex and assembly of siRNA into RNA-induced silencing complexes (RISC). Ago2 interacts with DICER1 through its Piwi domain. This Piwi domain is thought to provide RNA cleavage activity via a mechanism similar to RNase H. Ago2 activity is necessary for embryonic development as well as RNA-mediated gene silencing (RNAi).
Spécificité
human Ago2
Immunogène
Epitope: near the N-terminus of Ago2
KLH-conjugated, synthetic peptide containing the sequence YSGAGPALAPPAPPPPIQG
Application
Anti-Ago2 Antibody, clone 9E8.2 is a Mouse Monoclonal Antibody for detection of Ago2 also known as Argonaute-2, Eukaryotic translation initiation factor 2C & has been validated in WB and has been demonstrated to perform in ChIP and ChIP-seq.
ChIP and ChIP-seq: A representative lot of this antibody was used to perform ChIP and ChIP-seq ( Woolnough, JL, Atwood, BL, and Giles KE (2015), MCB Vol. 35 No. 13, p. 2278-2294).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
Chromatin Biology
RNA Binding Protein (RBP)
RNA Metabolism & Binding Proteins
Chromatin Biology
RNA Binding Protein (RBP)
Qualité
Routinely evaluated by immunoblot.
Description de la cible
~100 kda
Forme physique
Ascites with 0.05% sodium azide
ImmunoAffinity Purified
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Differential expression of microRNA expression in tamoxifen-sensitive MCF-7 versus tamoxifen-resistant LY2 human breast cancer cells.
Manavalan, TT; Teng, Y; Appana, SN; Datta, S; Kalbfleisch, TS; Li, Y; Klinge, CM
Cancer letters null
Li Zhang et al.
Cell death & disease, 10(3), 168-168 (2019-02-20)
Cholestasis induces the hepatic long non-coding RNA H19, which promotes the progression of cholestatic liver fibrosis. However, microRNAs that are dysregulated by H19 during cholestasis remain elusive. Using miRNA-sequencing analysis followed by qPCR validation, we identified marked upregulation of eight
Keriayn N Smith et al.
Stem cell reports, 9(1), 108-121 (2017-06-06)
Of the thousands of long noncoding RNAs expressed in embryonic stem cells (ESCs), few have known roles and fewer have been functionally implicated in the regulation of self-renewal and pluripotency, or the reprogramming of somatic cells to the pluripotent state.
Simon J Allison et al.
Molecular therapy. Nucleic acids, 2, e141-e141 (2014-01-09)
Selective gene silencing by RNA interference (RNAi) involves double-stranded small interfering RNA (ds siRNA) composed of single-stranded (ss) guide and passenger RNAs. siRNA is recognized and processed by Ago2 and C3PO, endonucleases of the RNA-induced silencing complex (RISC). RISC cleaves
Israel Pichardo-Casas et al.
Brain research, 1436, 20-33 (2011-12-27)
In recent years, microRNAs or miRNAs have been proposed to target neuronal mRNAs localized near the synapse, exerting a pivotal role in modulating local protein synthesis, and presumably affecting adaptive mechanisms such as synaptic plasticity. In the present study we
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