L040150

Cap 1

• Synonyme(s) : Cap A
• Code UNSPSC : 12352106
• Nomenclature NACRES : NA.21

Propriétés

• Type: for DNA synthesis
• Niveau de qualité: 200
• Gamme de produits: Proligo Reagents
• Forme: liquid
• Conditions de stockage: dry at room temperature
• Technique(s): DNA synthesis: suitable
• Impuretés: ≤200 ppm water content (Karl Fischer)
• Couleur: clear
• Adéquation: conforms to structure (GC, Formulation)
• Température de stockage: room temp

Description

Description générale

Cap 1 solution consists of n-methylimidazole, lutidine, and acetonitrile (ACN).

Application

Cap 1 is suitable for use after the phosphoramidite reaction/coupling step.

Actions biochimiques/physiologiques

Cap 1 solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Notes préparatoires

Acetonitrile / Lutidine / N-Methylimidazole 8/1/1 (v/v/v)

Informations sur la sécurité

• Pictogrammes: GHS02,GHS08,GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H225,H302 + H312 + H332,H314,H361d
• Conseils de prudence: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classification des risques: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Repr. 2 - Skin Corr. 1B
• Code de la classe de stockage: 3 - Flammable liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): 35.6 °F - closed cup
• Point d'éclair (°C): 2.0 °C - closed cup