861049

Azure A chloride

certified by the Biological Stain Commission, Dye content 70 %

• Synonyme(s) : Methylene blue
• Formule empirique (notation de Hill): C₁₄H₁₄ClN₃S
• Numéro CAS: 531-53-3
• Poids moléculaire : 291.80
• Numéro C.I. (Colour Index): 52005
• Numéro Beilstein : 3922287
• Numéro CE : 208-510-7
• Numéro MDL: MFCD00012112
• Code UNSPSC : 12171500
• ID de substance PubChem : 329769437
• Nomenclature NACRES : NA.47

Propriétés

• Qualité: certified by the Biological Stain Commission
• Niveau de qualité: 200
• Forme: powder
• Composition: Dye content, 70%
• Couleur: blue to very dark blue; very dark green
• Pf: 290 °C (dec.) (lit.)
• Solubilité: H₂O: 1 mg/mL
• λmax: 633 nm
• ε (coefficient d'extinction): ≥11000 at 242-246 nm in H₂O at -1; ≥26000 at 287-291 nm in H₂O; ≥34000 at 620-634 μm in H₂O
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: NC1=CC(SC(C2=N3)=CC(C=C2)=[N+](C)C)=C3C=C1.[Cl-]
• InChI: 1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
• Clé InChI: NALREUIWICQLPS-UHFFFAOYSA-N

Description

Application

Azure A has been used to determine the amount of charged dextran sulfate in the nanoparticles for the study involving incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles.

Actions biochimiques/physiologiques

The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye.

Adéquation

Certified for use in Lillie′s modified Nocht′s method and the NCCLS method for blood smears.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)