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R8508

Sigma-Aldrich

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)

buffered aqueous solution

Synonym(s):

Transfer RNA, tRNA

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352106
NACRES:
NA.55

grade

for molecular biology

Quality Level

form

buffered aqueous solution

concentration

9-11 mg/mL

foreign activity

DNase, Nickase, none detected

shipped in

dry ice

storage temp.

−20°C

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General description

Transfer RNA (tRNA) is isolated from baker′s yeast.
Transfer RNA (tRNA) is a short nucleotide RNA chain, which has an L-shaped structure.

Application

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used to precipitate mRNA of denuded oocytes and for reverse transcription. It has also been used to seal magnetic beads in RNA pull-down assay of nasopharyngeal carcinoma (NPC) cells.
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) is suitable for use as a carrier in nucleic acid purification and precipitation.

Biochem/physiol Actions

Transfer RNA (tRNA) acts as an ‘adaptor′ molecule that translates the three-nucleotide codon sequence in the mRNA to the corresponding amino acid of that codon. It determines the genetic code by linking amino acids and nucleic acids. tRNA is involved in viral replication, bacterial cell wall biosynthesis, cell stress response, and animal behavior control. Aberrations in tRNA molecules are associated with several human diseases.

Components

tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Encyclopedia of Agriculture and Food Systems (2015)
Patrick Lonergan et al.
Molecular reproduction and development, 66(3), 297-305 (2003-09-23)
In the cyclic cow, final maturation of the ovulatory follicle is initiated by the preovulatory luteinizing hormone (LH) surge. During the subsequent 24 hr period, the oocyte nucleus undergoes meiotic progression to metaphase II and several changes in cytoplasmic organization
Premal Shah et al.
Cell, 153(7), 1589-1601 (2013-06-26)
Deep sequencing now provides detailed snapshots of ribosome occupancy on mRNAs. We leverage these data to parameterize a computational model of translation, keeping track of every ribosome, tRNA, and mRNA molecule in a yeast cell. We determine the parameter regimes
Daniel Castro-Roa et al.
Nature chemical biology, 9(12), 811-817 (2013-10-22)
Fic proteins are ubiquitous in all of the domains of life and have critical roles in multiple cellular processes through AMPylation of (transfer of AMP to) target proteins. Doc from the doc-phd toxin-antitoxin module is a member of the Fic
Takamasa Hirano et al.
RNA (New York, N.Y.), 20(8), 1223-1237 (2014-06-11)
Small RNAs mediate gene silencing by binding Argonaute/Piwi proteins to regulate target RNAs. Here, we describe small RNA profiling of the adult testes of Callithrix jacchus, the common marmoset. The most abundant class of small RNAs in the adult testis

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