R3757
Reactive Yellow 3−Agarose
saline suspension
Synonym(s):
C.I.13245
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About This Item
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form
saline suspension
Quality Level
extent of labeling
1-5 mg per mL
matrix
cross-linked 4% beaded agarose
capacity
≥500 units/mL binding capacity (citrate synthase)
storage temp.
2-8°C
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Application
Reactive Yellow 3-agarose is used in affinity chromatography, protein chromatography and dye resins. Reactive Yellow 3-agarose has been used in immunohistochemical localization studies to show where tyramine N-(hydroxycinnamoyl)transferase (THT) polypeptides occur in opium poppy. Reactive Yellow-agarose has also been used to purify human cholesteryl ester transfer protein.
Physical form
Suspension in 0.5 M NaCl containing preservative
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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A T Dinkova-Kostova et al.
The Journal of biological chemistry, 271(46), 29473-29482 (1996-11-15)
Lignans are a widely distributed class of natural products, whose functions and distribution suggest that they are one of the earliest forms of defense to have evolved in vascular plants; some, such as podophyllotoxin and enterodiol, have important roles in
HPLC Assays for the Chorismate Utilizing Enzymes Chorismate Pyruvate Lyase and <I>Para</I>-Aminiobenzoate Synthase.
Bongaerts, R.J.M., and Verpoorte, R.
Journal of Liquid Chromatography and Related Technologies, 23(17), 2703-2713 (2000)
E F Rehberg et al.
Protein expression and purification, 5(3), 285-290 (1994-06-01)
Human cholesteryl ester transfer protein was purified from lipoprotein-depleted serum or plasma in a three-step procedure utilizing commercially available triazine dyes immobilized on agarose. The method used consisted of successive chromatography steps on Reactive Red 120 agarose (Procion Red H-E3B
Enzymes involved in hydroxycinnamate metabolism.
D Strack
Methods in enzymology, 335, 70-81 (2001-06-13)
M Yu et al.
Planta, 209(1), 33-44 (1999-08-31)
A development-specific and elicitor-inducible acyltransferase [hydroxycinnamoyl-CoA: tyramine N-(hydroxycinnamoyl)transferase (THT; EC 2.3.1.110)] that catalyzes the transfer of hydroxycinnamic acids from hydroxycinnamoyl-CoA esters to hydroxyphenethylamines was purified 988-fold to apparent homogeneity from opium poppy (Papaver somniferum L.) cell-suspension cultures. The purification procedure
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