G4410
Griess reagent (modified)
Synonym(s):
Greiss, nitrite assay
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General description
Griess reagent is based on Griess reaction. The components include naphthylethylenediamine dihydrochloride suspended in water and sulphanilamide in phosphoric acid. This reagent reacts with nitrite in samples to form a purple azo product, absorbance of which is measured at 546 nm.
Application
Griess reagent (modified) has been used:
- in the nitrite assay of primary spinal cord glial cells stimulated with supernatant of damaged sensory neuron (SDSN)
- for measuring nitrite/nitrate oxidized from nitric oxide in stimulated chondrocytes
- for measuring nitric oxide (NO) degradation in RAW264.7 macrophage cells
- to measure the concentration of nitric oxide(NO) in the conditioned media from cultured macrophages (MΦs)
- to determine the relative levels of NO released by BEAS-2B bronchial cells in the culture supernatants
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
WGK
WGK 2
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