C0412
Concanavalin A from Canavalia ensiformis (Jack bean)
Type IV-S, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture
Synonym(s):
Con A
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About This Item
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Quality Level
sterility
γ-irradiated
type
Type IV-S
product line
BioReagent
form
lyophilized powder
technique(s)
cell culture | mammalian: suitable
impurities
salt, essentially free
solubility
PBS: 5 mg/mL, slightly hazy
H2O: soluble, slightly hazy
storage temp.
−20°C
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Application
Concanavalin A from Canavalia ensiformis (jack bean) has been used:
- to induce spleen blastogenesis
- to generate ConA blast targets from B6 and lpr spleen cells
- to stimulate splenic lymphocytes from estrogen-and placebo-treated mice
Biochem/physiol Actions
Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Analysis Note
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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