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APOAF

Sigma-Aldrich

Annexin V-FITC Apoptosis Detection Kit

Synonym(s):

Annexin V-FITC, Apoptosis probe FITC

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.84

usage

(20 tests)

Quality Level

300

packaging

pkg of 1 kit

technique(s)

flow cytometry: suitable

application(s)

cell analysis
detection

detection method

fluorometric

shipped in

wet ice

storage temp.

2-8°C

General description

Annexin V-FITC kit allows fluorescent detection of annexin V bound to apoptotic cells and quantitative determination by flow cytometry. The AnnexinV-FITC kit uses annexin V conjugated with fluorescein isothiocyante (FITC) to label phosphatidylserine sites on the membrane surface. The kit includes propidium iodide (PI) to label the cellular DNA in necrotic cells where the cell membrane has been totally compromised. This combination allows the differentiation among early apoptotic cells (annexin V positive, PI negative), necrotic cells (annexin V positive, PI positive), and viable cells (annexin V negative, PI negative).

Application

Annexin V-FITC Apoptosis Detection Kit was used:
  • in staining of LNCaP prostate cancer cells for measuring the G. lucidum extracts activity during the treatment of prostate cancer.
  • for tumor cell labelling to study the inhibitory activity of DBP-maf (Vitamin D binding protein-macrophage activating factor) on prostate tumor cells.
  • for indirect measurement of flippase activity.

Features and Benefits

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL.
  • Rapid labeling of cells. Cell staining takes only 10 minutes.
  • No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
  • Propidium iodide secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Other Notes

Allow all components to reach room temperature before use.

Kit Components Only

Product No.
Description
  • APOAFA

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

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Certificates of Analysis (COA)

Lot/Batch Number
0000389724
0000378388
0000378389
0000378388
0000378389

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Denise C Arruda et al.
The Journal of biological chemistry, 287(18), 14912-14922 (2012-02-16)
Complementarity-determining regions (CDRs) from monoclonal antibodies tested as synthetic peptides display anti-infective and antitumor activities, independent of the specificity of the native antibody. Previously, we have shown that the synthetic peptide C7H2, based on the heavy chain CDR 2 from
Jianzhong Li et al.
Scientific reports, 6, 23975-23975 (2016-04-08)
Metformin, one of the most common prescriptions for patients with type 2 diabetes, is reported to protect the kidney from gentamicin-induced nephrotoxicity. However, the role and mechanisms for metformin in preventing cisplatin-induced nephrotoxicity remains largely unknown. In this study, a
Sanming Deng et al.
PloS one, 7(1), e30714-e30714 (2012-02-01)
The Crosstalk between a tumor and its hypoxic microenvironment has become increasingly important. However, the exact role of UCP2 function in cancer cells under hypoxia remains unknown. In this study, UCP2 showed anti-apoptotic properties in A549 cells under hypoxic conditions.
Kalvin J Gregory et al.
PloS one, 5(10), e13428-e13428 (2010-10-27)
Vitamin D binding protein-macrophage activating factor (DBP-maf) is a potent inhibitor of tumor growth. Its activity, however, has been attributed to indirect mechanisms such as boosting the immune response by activating macrophages and inhibiting the blood vessel growth necessary for
G Koopman et al.
Blood, 84(5), 1415-1420 (1994-09-01)
Apoptosis, or programmed cell death, is a general mechanism for removal of unwanted cells from the immune system. It is characterized by chromatin condensation, a reduction in cell volume, and endonuclease cleavage of DNA into oligonucleosomal length fragments. Apoptosis is
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