RSTREP-RO
Roche
Streptavidin, recombinant
liquid, ≥17 units/mg protein, pH range 5.0-9.0
Synonym(s):
streptavidin
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About This Item
UNSPSC Code:
12352202
Recommended Products
biological source
Streptomyces sp. ( Streptomyces avidinii)
Quality Level
recombinant
expressed in E. coli
form
liquid
specific activity
≥17 units/mg protein
mol wt
~52 kDa
packaging
pkg of 1 mg (11721666001)
pkg of 5 mg (11721674001)
manufacturer/tradename
Roche
concentration
70-90%
technique(s)
protein array: suitable
protein purification: suitable
impurities
6-7% water
pH range
5.0-9.0
pI
7.4-7.7 (two main bands)
solubility
water: 10 mg/L (mA405=100)
suitability
suitable for immunological tests
UniProt accession no.
shipped in
cooler
storage temp.
-10 to -25°C
General description
Streptavidin, a highly versatile protein, is naturally produced by Streptomyces avidinii. It exhibits a strong affinity for biotin, forming a non-covalent yet robust binding interaction. This unique property makes streptavidin an ideal choice for various biological detection systems, including western blotting, enzyme-linked immunosorbent assays (ELISA), and immunohistochemistry.
Application
Use Streptavidin, recombinant for coating of solid phases (e.g., microplates, beads etc.) as well with biotin-labeled haptens and antibodies in universal immunological detection systems (streptavidin/biotin system).
Sequence
Streptavidin is a tetramer with a molecular weight of approximately 53 kD consisting of four subunits of approximately 13 kD, each containing a single biotin-binding site.
Each subunit has six tyrosine residues.The protein is free of carbohydrate.
Each subunit has six tyrosine residues.The protein is free of carbohydrate.
Unit Definition
One unit binds 1 μg D-biotin at pH 8.9.
Physical form
White lyophilizate; lyophilized in a 20 mM potassium phosphate, pH 6.5 solution
Protein: 0.7 ± 0.1mg protein lyophilizate (A282 = 3.1)
KD: 10-15M
Protein: 0.7 ± 0.1mg protein lyophilizate (A282 = 3.1)
KD: 10-15M
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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