Skip to Content
Merck
  • Analysis of moniliformin in maize plants using hydrophilic interaction chromatography.

Analysis of moniliformin in maize plants using hydrophilic interaction chromatography.

Journal of agricultural and food chemistry (2007-10-27)
Jens Laurids Sørensen, Kristian Fog Nielsen, Ulf Thrane
ABSTRACT

A novel HPLC method was developed for detection of the Fusarium mycotoxin, moniliformin in whole maize plants. The method is based on hydrophilic interaction chromatography (HILIC) on a ZIC zwitterion column combined with diode array detection and negative electrospray mass spectrometry (ESI(-)-MS). Samples were extracted using acetonitrile-water (85:15), and the extracts were cleaned up on strong anion exchange columns. By this procedure we obtained a recovery rate of 57-74% moniliformin with a limit of detection at 48 ng/g and a limit of quantification at 96 ng/g using UV detection at 229 nm, which is comparable to current methods used. Limit of detection and quantification using ESI(-)-MS detection was 1 and 12 ng/g, respectively. Screening of maize samples infected with the moniliformin producing fungi F. avenaceum, F. tricinctum, or F. subglutinans detected moniliformin levels of 1-12 ng/g in 15 of 28 samples using ESI(-)-MS detection. To our knowledge this is the first example of HILIC separation in mycotoxin analysis.

MATERIALS
Product Number
Brand
Product Description

Supelco
Moniliformin sodium salt solution, ~100 μg/mL in acetonitrile: water, analytical standard
Sigma-Aldrich
Moniliformin sodium salt from Fusarium proliferatum