SRP4893

TFF1 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(s): BCEI, HP1, HPS, TFF-1, TFF1, pS2
• MDL number: MFCD07370822
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• mol wt: ~8.2 kDa
• packaging: pkg of 20 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• NCBI accession no.: P04155
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... TFF1(7031)

Description

General description

Trefoil factor 1(TFF1) is a member of trefoil factor family (TFF) and is encoded by the gene mapped to human chromosome 21q22.3. Recombinant human TFF1 is an 8.2kDa polypeptide containing 60 amino acid residues. TFF1 consists of trefoil motif, which is a 40-amino acid domain with three conserved disulfides. TFF1 is expressed and secreted by epithelial cells in the stomach or colon mucous membrane.

Biochem/physiol Actions

Trefoil factor family (TFF) members play a vital role in mucus stabilization and stimulation of normal epithelial cell restitution during wound healing via motogenic and antiapoptotic activities. TFF1 prevents anoikis, chemicals or bad-induced apoptosis by decreasing the caspase-3, caspase-6, caspase-8, and caspase-9 activities. TFF1 functions as a tumor suppressor in gastric cancer, whereas it acts as a tumor promoter in mucinous ovarian cancer via regulating the Wnt/β-catenin signaling pathway. Elevated level of TFF1 is observed in patients suffering from Chronic kidney disease (CKD). TFF1 might function as a biomarker for early detection of CKD.

Physical form

Sterile filtered and lyophilized powder.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers.