SRP0225

Rheb Active human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

• Synonym(s): GTP-binding protein Rheb, Ras homolog enriched in brain
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• Assay: ≥90% (SDS-PAGE)
• form: aqueous solution
• mol wt: 23 kDa
• packaging: pkg of 100 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_005614
• UniProt accession no.: Q15382

Description

General description

Human Rheb (GenBank Accession No. NM_005614), full length with N-terminal HIS6 tag, MW = 23 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of biochemical function.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.