SRP0170

ACK1 Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• Synonym(s): Activated Cdc42-associated kinase 1, TNK2, Tyrosine kinase, non-receptor, 2
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• Assay: ≥70% (SDS-PAGE)
• form: aqueous solution
• mol wt: 68 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_005781
• UniProt accession no.: Q07912
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... TNK2(10188)

Description

General description

ACK1 (activated CDC42 kinase 1) is a non-receptor tyrosine kinase and is present in all human tissues.[1] It belongs to the family of cytoplasmic tyrosine kinases. The protein has a sterile α motif (SAM) domain, a tyrosine kinase domain, a SH3 (src homology 3) domain, a CDC42/RAC-interactive (CRIB) domain, and a region that is homologous to the EGFR (epidermal growth factor receptor) binding domain of Mig6 (mitogen-induced gene 6) and a ubiquitin association domain.[2]
Human Ack1 (GenBank Accession No. NM_005781), full length (amino acids 110-476) with N-terminal GST tag, MW = 68kDa, expressed in Sf9 insect cell systems.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

ACK1 (activated CDC42 kinase 1) was first discovered for its binding to CDC42 (cell division control protein 42 homolog). ACK1 and CDC42 controls cellular attachment and migration.[1][2] ACK1 is involved in cell survival, proliferation and migration. It is also associated with various cancers.[3] The ACK1 gene is overexpressed in prostate, gastric and pancreatic cancers.[1] It also interacts with the heavy chain of clathrin and might be involved in receptor-mediated endocytosis.[4] ACK1 is a dual specificity kinase towards WASP (Wiskott–Aldrich Syndrome protein).[5] ACK1 is also responsible for the phosphorylation and activation of DBL (an exchange factor for Rho) and Ras/GRF1 (an exchange factor for Ras).[6]

Unit Definition

One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substrate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.