As per the product information sheet, 100% Ethanol is suggested for the dilution of the wash solution. The use of 100% methanol has not been validated and is not recommended for the isolation of RNA. Kindly review the product information sheet available at this link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/188/017/snc10bul.pdf
SNC50
mirPremier® microRNA Isolation Kit
1 sufficient for 50 preparations
Synonym(s):
microRNA Isolation Kit
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About This Item
UNSPSC Code:
41105501
NACRES:
NA.52
Pricing and availability is not currently available.
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General description
Sigma′s mirPremier microRNA Isolation Kit provides a rapid and efficient method for purifying and enriching miRNAs and other small RNAs from diverse biological sources, including mammalian cell cultures, animal tissues, plant tissues, and microbial cultures, without using hazardous organic extractions. microRNAs (miRNAs) are a class of small RNA molecules, about 21 nucleotides (nt) in length, that regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. In addition, the kit also can be used for isolating total RNA if messenger RNA or other large RNAs are of interest.
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Application
Features and Benefits
- Designed to enhance the efficiency of isolating microRNA and other small RNA molecules directly from a wide range of biological sources.
- Enables fast and efficient extraction and concentration of miRNA in 30 minutes for downstream applications.
- Can extract high-purity miRNA with no detectable large RNA
- No dangerous organic extractions are involved.
Legal Information
mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany
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Description
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Interspecies communication between plant and mouse gut host cells through edible plant derived exosome-like nanoparticles.
Mu J
Molecular Nutrition And Food Research, 58(7), 1561-1573 (2014)
Abdulla Abdulla Sabana et al.
Planta, 251(4), 79-79 (2020-03-14)
Genome-wide analysis of small RNAs identifies somatic embryogenesis- specific miRNAs and their targets and provides novel insights into the mechanisms governing somatic embryogenesis in coconut, a highly in vitro recalcitrant species. Coconut, a major plantation crop of the tropics is
Jon Bråte et al.
Current biology : CB, 28(20), 3288-3295 (2018-10-16)
The emergence of multicellular animals was associated with an increase in phenotypic complexity and with the acquisition of spatial cell differentiation and embryonic development. Paradoxically, this phenotypic transition was not paralleled by major changes in the underlying developmental toolkit and
Dongxiao Su et al.
Food & function, 8(2), 808-815 (2017-01-26)
Dietary phenolics exhibit hypolipidemic activity by changing lipid metabolism-related microRNA (miRNA) expression. Quercetin 3-O-rutinoside-7-O-α-l-rhamnosidase (quercetin 3-rut-7-rha), rutin and (-)-epicatechin are the main phenolics in lychee (Litchi chinensis Sonn.) pulp. A previous study reported that quercetin 3-rut-7-rha and rutin had hypolipidemic
Unicellular origin of the animal microRNA machinery
Br?te J, et al.
Current Biology, 28(20), 3288- 3295 (2018)
Articles
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
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Methanol 100% can be used for the dilution of the wash solution instead of ethanol 100%?
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Can I purify 18S and 28S large RNAs along with miRNAs with the mirPremier® microRNA Isolation Kit?
1 answer-
Large RNAs (18S and 28S) from the pellet fraction can be purified after transferring the microRNA-containing supernatant to a new tube by using the total RNA protocol or by phenol/chloroform extraction.
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How can I avoid co-purifying mRNAs and rRNAs when purifying small RNAs from gram negative bacteria using mirPremier® microRNA Isolation Kit?
1 answer-
Too much residual medium or cell mass may lead to recovery of some large RNAs. It is critical to remove as much residual medium as possible by re-centrifuging the pellet. One can also test different ratios of the lysis mix (Small RNA Lysis Buffer and Binding Solution).
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Can the mirPremier® microRNA Isolation Kit be used to isolate small RNAs from purified total RNA?
1 answer-
We have used to the kit to purify in vitro transcribed small RNAs with great success, but have not done so with purified total RNA. Here are the steps we would recommend trying with purified total RNA:1. Prepare a lysis mix with 0.7 vol. Small RNA Lysis Buffer (M1070) and 0.3 vol. Binding Solution (L8042).2. Add 500 ul of lysis mix with 50 ul total RNA and mix thoroughly.3. Spin at 14000 rpm for 5 min to precipitate large RNA.5. Transfer the supernatant to a new tube.6. Add 610 ul (1.1 vol.) 100% ethanol to the supernatant and mix well.7.Transfer the mixture to a binding column and spin 1 min to bind. Repeat the binding step with the remaining mixture.8. Wash the column first with 700 ul 100% ethanol, and then with ethanol-diluted wash Solution 2.9. Dry the column and elute small RNA.
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Has the mirPremier® microRNA Isolation Kit been tested on sperm cells?
1 answer-
We have not tested mirPremier™ microRNA Isolation Kit with sperm cells.
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