SAE0180
3CL, Mpro protease fluorogenic substrate peptide
AFC-Peptide substrate, ≥95% (HPLC), solid
Synonym(s):
3CL protease substrate
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product name
3CL, Mpro protease fluorogenic substrate peptide, AFC-Peptide substrate, lyophilized
Quality Level
Assay
≥95% (HPLC)
form
solid
shipped in
ambient
storage temp.
−20°C
General description
This fluorogenic peptide is a substrate for the Main protease from SARS-Cov2, 3CL protease (Mpro). This substrate allows measurement of the activity of 3CL protease using a standard plate reader or spectrophotometer. Proteolytic cleavage by 3CL protease will release the fluorescent AFC group from the peptide (sequence TSAVLQ). After release, an increase in fluoresence is obtained (Excitation = 400 nm, Emission = 505 nm).
Peptide: Thr-Ser-Ala-Val-Leu-Gln-AFC
Lyophilized from 0.1% TFA in H2O
Peptide: Thr-Ser-Ala-Val-Leu-Gln-AFC
Lyophilized from 0.1% TFA in H2O
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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