Skip to Content
Merck
All Photos(2)

Key Documents

View All Documentation

SAB4200376

Sigma-Aldrich

Anti-Atg13 antibody, Mouse monoclonal

clone ATG13-8, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Atg13 antibody produced in mouse, Monoclonal Anti-Autophagy related 13 homolog (S. cerevisiae), Monoclonal Anti-KIAA0652

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

ATG13-8, monoclonal

form

buffered aqueous solution

mol wt

antigen ~65 kDa

species reactivity

human, mouse, rat

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using lysates of mouse LA-4 cells.
western blot: 1-2 μg/mL using whole extracts of HEK-293T cells over expressing human Atg13.

isotype

IgG2a

UniProt accession no.

O75143

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ATG13(9776)
mouse ... Atg13(51897)
rat ... Atg13(362164)

General description

Monoclonal Anti-ATG13 (mouse IgG2a isotype) is derived from the hybridoma ATG13-8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice.

Immunogen

synthetic peptide corresponding to the C-terminal region of human ATG13, conjugated to KLH. The corresponding sequence is identical in mouse and rat Atg13.

Application

Monoclonal Anti-Atg13 antibody produced in mouse has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Atg13 is essential for autophagosome formation in mammalian cells. Atg13 forms a stable complex with UNC-51-like kinase 1 (ULK1) and family-interacting protein of 200 kDa (FIP200).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
081M4809

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy
Hosokawa N, et al.
Molecular Biology of the Cell, 20(7), 1981-1991 (2009)
Tomokazu Murakawa et al.
Cell reports, 26(2), 338-345 (2019-01-10)
Degradation of mitochondria by selective autophagy, termed mitophagy, contributes to the control of mitochondrial quality. Bcl2-L-13 is a mammalian homolog of Atg32, which is an essential mitophagy receptor in yeast. However, the molecular machinery involved in Bcl2-L-13-mediated mitophagy remains to

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service