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RAB0224

Sigma-Aldrich

Mouse IFN-γ ELISA Kit

for serum, plasma, cell culture supernatant

Synonym(s):

IFN-gamma, Interferon gamma

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 pg/mL
standard curve range: 2.74-2000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Ifng(15978)

General description

The Mouse IFN-γ ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IFN-γ in serum, plasma and cell culture supernatants.

Immunogen

Recombinant Mouse IFN-γ

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Mouse IFN-γ ELISA Kit has been used to measure serum proinflammatory cytokine levels in a renal ischemia/reperfusion injury model.

Biochem/physiol Actions

Interferon γ (IFN-γ) regulates cell differentiation and inflammatory responses. It stimulates the expression of apoptotic mediators. IFN-γ is associated with rheumatoid arthritis (RA). It reduces the differentiation of T helper (Th) 17 cells and osteoclasts. IFN-γ has immunomodulatory and antiviral activity. It is associated with autoimmune pathology. IFN-γ provides protection against tumor development.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Interferon-gamma regulates inflammatory cell death by targeting necroptosis in experimental autoimmune arthritis
Lee SH, et al.
Scientific Reports, 7(1), 10133-10133 (2017)
Interferon-gamma: an overview of signals, mechanisms and functions
Schroder K, et al.
Journal of Leukocyte Biology, 75(2), 163-189 (2004)
Qianqian Chen et al.
Journal of photochemistry and photobiology. B, Biology, 216, 112152-112152 (2021-02-21)
Emerging evidence suggests that the gut microbiota plays an important role in the pathological progression of Alzheimer's disease (AD). Photobiomodulation (PBM) therapy is believed to have a positive regulatory effect on the imbalance of certain body functions, including inflammation, immunity
K Kim et al.
Tuberculosis (Edinburgh, Scotland), 94(5), 494-501 (2014-08-07)
Previous studies suggest that control of Mycobacterium tuberculosis infection is compromised by the activity of regulatory T cells, including those that express CD39, an ectonucleotidase with immunosuppressive properties. Here, we examine the role of CD39 on CD4+ T cells reacting
Kaveh Abdi et al.
Journal of immunology (Baltimore, Md. : 1950), 192(12), 6028-6036 (2014-05-14)
IL-12p40 partners with the p35 and p19 polypeptides to generate the heterodimeric cytokines IL-12 and IL-23, respectively. These cytokines play critical and distinct roles in host defense. The assembly of these heterodimers is thought to take place within the cell
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