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R3400

Sigma-Aldrich

Monoclonal Anti-c-K-Ras antibody produced in mouse

clone 234-4.2, purified immunoglobulin, buffered aqueous solution

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About This Item

MDL number:
MFCD00239650
UNSPSC Code:
12352203

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

234-4.2, monoclonal

form

buffered aqueous solution

mol wt

antigen 21 kDa

species reactivity

mouse, human, rat

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10 μg/mL using normal skin tissue
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2κ

UniProt accession no.

P01116

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... KRAS(3845)
mouse ... Kras(16653)
rat ... Kras(24525)

General description

c-K-Ras or KRAS (kirsten rat sarcoma-2 virus oncogene) is a small GTP-binding protein. It is located on human chromosome 12p12.1. KRAS has two splice variants, such as KRASA and KRASB (KRAS proto-oncogenes). Expression of KRASA is restricted to specific tissues whereas KRASB is expressed everywhere .

Specificity

The antibody reacts with c-K-Ras and v-K-Ras p21. It crossreacts with cH-Ras and cN-Ras p21 on immunoblots.

Immunogen

recombinant p21 protein

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

KRAS (kirsten rat sarcoma-2 virus oncogene) mutation leads to poor prognosis of early rectal cancer.

Physical form

Solution in 0.05 M sodium phosphate buffer, pH7.5, containing 0.2% gelatin and < 0.1% sodium azide

Preparation Note

Purified using protein A or G

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jasminka Omerovic et al.
The Biochemical journal, 426(1), 65-72 (2009-11-20)
Oncogenic Ras mutations render the protein constitutively active and promote tumorigenesis via chronic stimulation of effector pathways. In A549 lung adenocarcinoma approx. 50% of the total Ras population is constitutively active, yet these cells display only weak activation of the
David A Iglesias et al.
Molecular cancer therapeutics, 12(12), 2847-2856 (2013-10-01)
Metformin is an oral biguanide commonly used for the treatment of type II diabetes and has recently been demonstrated to possess antiproliferative properties that can be exploited for the prevention and treatment of a variety of cancers. The mechanisms underlying
Candida Zuchegna et al.
Redox report : communications in free radical research, 27(1), 150-157 (2022-07-14)
Although the protooncogenes small GTPases Ras are redox-sensitive proteins, how they are regulated by redox signaling in the central nervous system (CNS) is still poorly understood. Alteration in redox-sensitive targets by redox signaling may have myriad effects on Ras stability
Harrison J Hocker et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(25), 10201-10206 (2013-06-06)
Aberrant signaling by oncogenic mutant rat sarcoma (Ras) proteins occurs in ∼15% of all human tumors, yet direct inhibition of Ras by small molecules has remained elusive. Recently, several small-molecule ligands have been discovered that directly bind Ras and inhibit
Kwang-jin Cho et al.
The Journal of biological chemistry, 287(52), 43573-43584 (2012-11-06)
Oncogenic mutant Ras is frequently expressed in human cancers, but no anti-Ras drugs have been developed. Since membrane association is essential for Ras biological activity, we developed a high content assay for inhibitors of Ras plasma membrane localization. We discovered
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