Product P8287, o-Phenylenediamine dihydrochloride tablet will work, but it will require the addition of phosphate-citrate buffer and hydrogen peroxide. Suggested part numbers for these additional components are included in the datasheet: https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/5/p8287dat.pdf
P9187
OPD
SIGMAFAST™, peroxidase substrate, chromogenic, tablet
Synonym(s):
OPD Peroxidase Substrate
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
Pricing and availability is not currently available.
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Product Name
SIGMAFAST™ OPD, tablet
form
tablet
Quality Level
solubility
water: 0.5 tablet/10 mL, clear, colorless
storage temp.
2-8°C
Application
SIGMAFAST™ OPD (o-Phenylenediamine dihydrochloride) tablets have been developed for use as a soluble substrate for the detection of peroxidase activity in enzyme immunoassays (EIA). OPD is the EIA substrate of choice as it exhibits high sensitivity. EIA applications utilizing OPD may be read in timed assays or stopped with dilute acid solutions for delayed readings.
SIGMAFAST OPD has been used in saliva- virus-like particles (VLP) binding assay.[1] It has also been used to develop enzyme linked immunosorbent assay (ELISA) plates for the detection of rituximab in rat serum samples.[2]
SIGMAFAST OPD has been used in saliva- virus-like particles (VLP) binding assay.[1] It has also been used to develop enzyme linked immunosorbent assay (ELISA) plates for the detection of rituximab in rat serum samples.[2]
Features and Benefits
SIGMAFAST™ OPD Tablets require no additional buffers or steps to prepare an active substrate solution.
Caution
This substrate is hygroscopic and light sensitive
Reconstitution
Each tablet set dissolved in 20 mL deionized water yields a ready-to-use buffered solution containing OPD and urea hydrogen peroxide.
One OPD tablet and one urea hydrogen peroxide/buffer tablet, dissolved in 20 ml of water, provides 20 ml of ready-to-use substrate. When the tablet is dissolved in this manner, the final concentrations will be 0.4 mg/ml OPD, 0.4 mg/ml urea hydrogen peroxide, and 0.05 M phosphate-citrate, pH 5.0. Tablets are individually packaged in foil packets.
Legal Information
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Skin Sens. 1 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
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Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Protocols
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
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What is an alternative for the SIGMAFAST OPD tablet set?
1 answer-
Helpful?
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Can I make the solution in large quantity and keep it in the fridge? Or should the solution be used right away because of its light sensitivity?
1 answer-
The Substrate Solution is light-sensitive and should be protected from direct sunlight or UV sources in a
tightly capped amber bottle. For best results, the Substrate Solution should be used within one hour.The Product Information Sheet is available for additional details:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/671/455/p9187pis-ms.pdfHelpful?
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