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P5493

Sigma-Aldrich

Phosphate buffered saline

10X, solution, pH 7.2-7.6 (25 °C), BioPerformance Certified, for molecular biology

Synonym(s):

PBS

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About This Item

MDL number:
MFCD00131855
UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25
Pricing and availability is not currently available.

Product Name

Phosphate buffered saline, 10× concentrate, BioPerformance Certified, suitable for cell culture

grade

BioPerformance Certified
for molecular biology

Quality Level

100
200

sterility

0.2 μm filtered

form

solution

storage condition

(Tightly closed)

technique(s)

cell culture | mammalian: suitable
immunocytochemistry: suitable
immunofluorescence: suitable

impurities

Bioburden, tested
 endotoxin, tested
≤5 ppm heavy metals (as Pb)

color

colorless

pH

7.2-7.6 (25 °C)

solubility

water: soluble

cation traces

heavy metals (as Pb): ≤5 ppm

suitability

suitable for
suitable for flow cytometry
suitable for microscopy

application(s)

advanced drug delivery

foreign activity

DNAse, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected

SMILES string

OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]

InChI

InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5

InChI key

LOKCTEFSRHRXRJ-UHFFFAOYSA-I

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Application

Phosphate buffered saline has been used:
  • as a cell wash solution in human dental pulp stem cells (DPSCs)[1]
  • for perfusion studies in dissected brain samples[2]
  • as wash solution for neurons culture cells sample preparation for immunofluorescence and immunocytochemical labelling[3]
  • as wash solution for ovary and sperm during in vitro culture and fertilization[4]

Packaging

4L package size comes in a box with a spigot for spill free dispensing.

Other Notes

When diluted to a 1X concentration, this product will yield a phosphate buffered saline solution with a phosphate buffer concentration of 0.01M and a sodium chloride concentration of 0.154M. The solution pH will be 7.4.
For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000345498
0000362469
0000362469
0000332509
0000334771

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Differences in serum steroid hormones concentrations in spontaneously hypertensive
Kozlowska A, et al.
Physiological Research (2018)
Maturation, Fertilization and Implantation of Cattle Ova Cultured in Different Media
Saif AH, et al.
The eurasia proceedings of science, technology, engineering & mathematics., 3(9), 181-188 (2018)
Stephen J Bush et al.
G3 (Bethesda, Md.), 9(2), 359-373 (2018-12-12)
One of the most significant physiological challenges to neonatal and juvenile ruminants is the development and establishment of the rumen. Using a subset of RNA-Seq data from our high-resolution atlas of gene expression in sheep (Ovis aries) we have provided
Diverse effect of BMP-2 homodimer on mesenchymal progenitors of different origin
Hrubi E, et al.
Human Cell : Official Journal of Human Cell Research Society, 31(2), 139-148 (2018)
Distribution and Neurochemistry of the Porcine Ileocaecal Valve Projecting Sensory Neurons in the Dorsal Root Ganglia and the Influence of Lipopolysaccharide from Different Serotypes of Salmonella spp. on the Chemical Coding of DRG Neurons in the Cell Cultures
Mikolajczyk A, et al.
International Journal of Molecular Sciences, 19(9), 2551-2551 (2018)
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Questions

1–8 of 8 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. What is the molecular weight of Product No. P5493 (Phosphate buffered saline)?

    1 answer

    1. Product P5493 is a 10× concentrate phosphate buffered saline solution, certified for bio-performance and suitable for cell culture. This solution comprises two primary ingredients diluted in water:

      - Disodium hydrogenorthophosphate with a molecular weight of 141.96 g/mol, identified by CAS number 7558-79-4.
      - Sodium chloride with a molecular weight of 58.44 g/mol, identified by CAS number 7647-14-5.

      Helpful?

  4. What is the formulation of the 10X PBS, P5493?

    1 answer

    1. The exact formulation for this 10X PBS product remains proprietary. It does not contain calcium, magnesium, or glucose. Per the product page: When diluted to a 1X concentration, this product will yield a phosphate buffered saline solution with a phosphate buffer concentration of 0.01M and a sodium chloride concentration of 0.154M. The solution pH will be 7.4.

      Helpful?

  5. What is the concentration of KCl in 10x PBS? Which phosphate salts are used and what is the concentration of each salt?

    1 answer

    1. Please see the responses to each questions below:

      Q: What is the concentration of KCl in 10x PBS?
      A: No KCl is used. P5493 contains NaCl.

      Q: Which phosphate salts are used and what is the concentration of each salt?
      A: This is specifically a sodium phosphate buffer, not a mixture of sodium phosphate and potassium phosphate buffer. Unfortunately, the detailed composition remains proprietary.

      Helpful?

  6. Does Product P5493, Phosphate buffered saline (PBS), contain calcium and magnesium?

    1 answer

    1. No, it does not contain calcium and magnesium.

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. What is the concentration of the 10X stock solution of Product P5493, Phosphate buffered saline (PBS)?

    1 answer

    1. As formulated, the 10X solution is 100 mM phosphate and 1.54 M sodium chloride.

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