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P0430

Sigma-Aldrich

Periodic acid

suitable for electrophoresis, ≥99%

Synonym(s):

Iodic acid, Orthoperiodic acid, Paraperiodic acid

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About This Item

Empirical Formula (Hill Notation):
H5IO6
CAS Number:
10450-60-9
Molecular Weight:
227.94
EC Number:
233-937-0
MDL number:
MFCD00011344
UNSPSC Code:
41105319
PubChem Substance ID:
24898104
NACRES:
NB.22
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Quality Level

100

Assay

≥99%

form

powder or crystals

reaction suitability

reagent type: oxidant

technique(s)

electrophoresis: suitable

mp

122 °C (lit.)

solubility

water: 100 mg/mL, clear, colorless

SMILES string

OI(O)(O)(O)(O)=O

InChI

1S/H5IO6/c2-1(3,4,5,6)7/h(H5,2,3,4,5,6,7)

InChI key

TWLXDPFBEPBAQB-UHFFFAOYSA-N

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Application

Periodic acid is commonly used as a component for glycoprotein stains[1]. Selective staining of glycoproteins on SDS-PAGE and membranes is carried out using a modification of the Periodic Acid-Schiff (PAS) method. Staining of sugar moieties of glycoproteins yields magenta bands with a colorless background. The Periodic Acid/Schiff Reagent stains vicinal diol groups found mainly on peripheral sugars and sialic acids and is used as a general glycoprotein stain[2]. Periodic acid is also used to cleave reversible cross-linkers (e.g. DATD, DHEBA) in PAGE gels.

Analysis Note

Tested for use in staining of glycoproteins.

Signal Word

Danger

Hazard Statements

H271,H314,H372,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Ox. Sol. 1 - Skin Corr. 1B - STOT RE 1 Oral

Target Organs

Thyroid

Storage Class Code

5.1A - Strongly oxidizing hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL7085
BCCH5285
BCCG6110
BCCG4583
BCCF1091

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Identification of glycoproteins on nitrocellulose membranes and gels.
D J Thornton et al.
Methods in molecular biology (Clifton, N.J.), 32, 119-128 (1994-01-01)
Glycoprotein staining following electrophoresis on acrylamide gels.
R M Zacharius et al.
Analytical biochemistry, 30(1), 148-152 (1969-07-01)
Aranzazu Villasante et al.
Biomaterials, 35(22), 5785-5794 (2014-04-22)
Monolayer cultures of tumor cells and animal studies have tremendously advanced our understanding of cancer biology. However, we often lack animal models for human tumors, and cultured lines of human cells quickly lose their cancer signatures. In recent years, simple
D Canbaz et al.
Allergy, 70(5), 522-532 (2015-02-14)
The initial immune response to house dust mite (HDM) is orchestrated by an interplay between epithelial cells (ECs) and dendritic cells (DCs). Innate cytokines released by HDM-exposed ECs activate airway DCs and effector inflammatory cells, which together induce a HDM-specific
Vaibhav Agarwal et al.
Journal of immunology (Baltimore, Md. : 1950), 191(8), 4235-4245 (2013-09-17)
Streptococcus pneumoniae (pneumococcus) is a major human pathogen, which evolved numerous successful strategies to colonize the host. In this study, we report a novel mechanism of pneumococcal-host interaction, whereby pneumococci use a host complement protein C1q, primarily involved in the
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