N9389

4-Nitrophenyl phosphate disodium salt hexahydrate

chromogenic, tablet

• Synonym(s): p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate
• Linear Formula: O₂NC₆H₄OP(O)(ONa)₂ · 6H₂O
• CAS Number: 333338-18-4
• Molecular Weight: 371.14
• Beilstein: 5324661
• EC Number: 224-246-5
• MDL number: MFCD00066288
• UNSPSC Code: 12352204
• PubChem Substance ID: 24897898
• NACRES: NA.83

Properties

• product name: 4-Nitrophenyl phosphate disodium salt hexahydrate, tablet
• description: 5 mg substrate per tablet
• form: tablet
• fluorescence: λ_ex 310 nm (pH 7.1); λ_ex 399 nm (Reaction Product)
• storage temp.: −20°C
• SMILES string: O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
• InChI: 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
• InChI key: KAKKHKRHCKCAGH-UHFFFAOYSA-L

Description

General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used in:

• enzyme-linked immunosorbent assay (ELISA) to visualize glycoprotein VI (GPVI) binding to collagen type I (Col I)
• enzymatic color reaction for semi-quantitative measurement of allergen-specific IgE by ELISA
• alkaline phosphatase assay

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves