It is recommended to homogenize cells using mechanical cell disruption in water only, as common buffer components can interfere with the protein carbonyl content of the sample. If the protein is very dilute, it can be concentrated using a 10 kDa spin filter. For the assay, it is advised to use 100 µL of sample containing approximately 0.5-2 mg protein and include a reagent background control by using 100 µL of dH2O alone. Regarding the impact of xylenes on this assay, it would need to be confirmed empirically.
MAK094
Protein Carbonyl Content Assay Kit
sufficient for 100 colorimetric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.
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usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
cardiovascular diseases; neurological disorders; cancer; gastrointestinal diseases
storage temp.
2-8°C
General description
Oxidative stress results when the effectiveness of antioxidant defenses is insufficient to deal with the production of reactive oxygen species (ROS). ROS can induce damage to DNA, lipids, and proteins. The oxidation of proteins results in the production of stable carbonyl groups, which can be used as a measure of oxidative injury.
Application
Suitability
Suitable for quantifying carbonyls in protein samples
Principle
The Protein Carbonyl Content Assay Kit provides a simple and direct procedure for measuring carbonyl content in a variety of biological samples. Carbonyl content is determined by the derivatization of protein carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH) leading to the formation of stable dinitrophenyl (DNP) hydrazone adducts, which can be detected spectrophotometrically at 375 nm, proportional to the carbonyls present.
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Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
8A - Combustible corrosive hazardous materials
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Could you please provide information on the type of buffer to use for isolating proteins from insect tissue in a way that will preserve the carbonylation for the MAK094-1KT Protein Carbonyl Content Assay Kit? Additionally, do you anticipate any adverse effects from Decolor paint pens on the cuticle of the insect tissue sample?
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